临床腹泻猪空肠组织中肾素-血管紧张素系统(RAS)的表达变化及与肠道炎症的关系

doi:10.11843/j.issn.0366-6964.2024.02.032

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (2): 751-758.doi: 10.11843/j.issn.0366-6964.2024.02.032

临床腹泻猪空肠组织中肾素-血管紧张素系统(RAS)的表达变化及与肠道炎症的关系

陈雪清¹, 李志强¹, 吴雨龙², 张崇昊¹, 张源淑^1*

1. 南京农业大学农业部动物生理生化重点开放实验室, 南京 210095;
2. 南京晓庄学院食品科学学院, 南京 211171

收稿日期:2023-02-01 出版日期:2024-02-23 发布日期:2024-02-27
通讯作者: 张源淑,主要从事动物营养和肠道健康研究,E-mail:zhangyuanshu@njau.edu.cn
作者简介:陈雪清(1999-),女,内蒙古呼和浩特人,硕士,主要从事动物营养和肠道健康研究,E-mail:chenxueqing1225@163.com
基金资助:
国家自然科学基金(31972640)

Expression of Renin Angiotensin System (RAS) in Jejunum Tissues of Piglets with Clinical Diarrhea and Its Relationship with Intestinal Inflammation

CHEN Xueqing¹, LI Zhiqiang¹, WU Yulong², ZHANG Chonghao¹, ZHANG Yuanshu^1*

1. Key Laboratory of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China;
2. School of Food Science, Nanjing Xiaozhuang University, Nanjing 211171, China

Received:2023-02-01 Online:2024-02-23 Published:2024-02-27

摘要/Abstract

摘要： 在现代生猪规模化养殖过程中,无论是断奶、换舍和换料等因素引起的应激反应还是细菌、病毒感染都极易影响消化道健康引起以腹泻为主要症状的肠道炎症。本文通过研究临床腹泻猪空肠组织中肾素-血管紧张素系统(renin-angiobensin system, RAS)的表达变化,探讨其与肠道炎症的关系。以临床上出现腹泻症状的保育猪为研究对象,截取其空肠组织,采用组织病理学、Western blot和RT-qPCR等方法,观察其空肠组织病理变化,明确RAS组分特别是血管紧张素转化酶2(angiotensin-converting enzyme 2,ACE2)的表达变化,探究其对猪肠道炎症损伤的影响。结果表明,临床腹泻保育猪的空肠组织中促炎性因子TNF-α、IL-1β显著升高,抗炎性因子IL-10无明显变化;组织病理学显示,空肠上皮绒毛脱落,腺体增生,存在大量出血点;ACE2、MasR mRNA和蛋白水平及Ang(1-7)含量均降低,ACE和AT1R蛋白水平及Ang II含量均升高。结果提示,临床腹泻保育猪空肠组织局部RAS被激活,ACE-Ang II-AT1R轴的活性远高于ACE2-Ang(1-7)-MasR轴,ACE2抗炎性损伤作用处于劣势。

关键词: 肾素-血管紧张素系统, 保育猪, 空肠, 临床腹泻, 肠道炎症

Abstract: In the process of modern large-scale pig breeding, whether it is the stress response caused by weaning, replacement and replacement, or bacterial and viral infections, it is very easy to affect the health of the digestive tract and cause intestinal inflammation with diarrhea as the main symptom. The expression of renin-angiotensin system (RAS) in jejunum tissues of pigs with clinical diarrhea was studied to explore the relationship between RAS and intestinal inflammation. The study was conducted on nursery pigs with clinical symptoms of diarrhea, and the pathological changes of jejunum tissues of diarrhea pigs were observed by histopathology, Western blot and RT-qPCR. To clarify the expression changes of RAS components, especially angiotensin-converting enzyme 2(ACE2), and explore the effects of RAS on intestinal inflammation injury in pigs. The results showed that the pro-inflammatory factors TNF-α and IL-1β were significantly increased in jejunum tissues of pigs with clinical diarrhoea piglets, while the anti-inflammatory factor IL-10 was not significantly changed. Histopathology showed that jejunum epithelial villi exfoliated, glandular hyperplasia and hemorrhagic sites. The mRNA and protein levels of ACE2 and MasR and the content of Ang (1-7) were decreased, while the protein levels of ACE and AT1R and the content of Ang II were increased. The results suggest that local jejunum RAS is activated in the jejunal tissue of pigs with clinical diarrhoea piglets. The activity of ACE-Ang II-AT1R axis was much higher than that of ACE2-ang (1-7)-MasR axis, and ACE2 was inferior in anti-inflammatory damage.

Key words: renin-angiotensin system, piglets, jejunum, clinical diarrhea, intestinal inflammation

中图分类号:

S852.23

陈雪清, 李志强, 吴雨龙, 张崇昊, 张源淑. 临床腹泻猪空肠组织中肾素-血管紧张素系统(RAS)的表达变化及与肠道炎症的关系[J]. 畜牧兽医学报, 2024, 55(2): 751-758.

CHEN Xueqing, LI Zhiqiang, WU Yulong, ZHANG Chonghao, ZHANG Yuanshu. Expression of Renin Angiotensin System (RAS) in Jejunum Tissues of Piglets with Clinical Diarrhea and Its Relationship with Intestinal Inflammation[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 751-758.

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临床腹泻猪空肠组织中肾素-血管紧张素系统(RAS)的表达变化及与肠道炎症的关系

Expression of Renin Angiotensin System (RAS) in Jejunum Tissues of Piglets with Clinical Diarrhea and Its Relationship with Intestinal Inflammation

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