文昌鸡性成熟启动前后下丘脑c-Myc/LIN28B/let-7a通路表达规律分析

doi:10.11843/j.issn.0366-6964.2016.04.010

摘要/Abstract

摘要：

本研究旨在分析c-Myc/LIN28B/let-7a调节通路与鸡性成熟启动的关联。测定5~17周龄文昌鸡母鸡卵巢重量、最大卵泡直径、输卵管长度及冠大小的发育性变化，并采用qRT-PCR检测c-Myc/LIN28B/let-7a基因在性成熟启动前后下丘脑中的表达。结果表明，在发育早期，文昌鸡性腺组织发育缓慢，卵泡保持原始状态。12周龄后，性腺组织进入快速发育期，13周龄母鸡的卵巢重量（1.35 g）和输卵管长度（15.68 cm）与12周龄测量值（0.60 g，7.16 cm）相比分别增加了125%和120%，同时出现等级前小白卵泡（SWF，直径1.0~2.0 mm）或大白卵泡（LWF，直径3.0~5.0 mm），由此确定，12~13周龄为文昌鸡母鸡的性成熟启动关键时机。qRT-PCR检测表明，c-Myc、LIN28B基因在文昌鸡性成熟启动时，下丘脑组织中的表达水平显著降低（P<0.05，P<0.01），且c-Myc基因的低表达能够持续到开产，而LIN28B基因表达水平在开产前（15周龄）又恢复至较高水平；性成熟启动后let-7a表达水平显著升高（P<0.01），并持续至开产。综上表明，c-Myc→LIN28B┤let-7a通路参与鸡性成熟启动调节。

关键词: 文昌鸡, 性成熟启动, 下丘脑, 基因表达

Abstract:

This experiment was conducted to investigate the role of c-Myc/LIN28B/let-7a pathway in chicken puberty onset.The Wenchang chicken breed was used as the material.The comb size，ovary weight，oviduct length and follicle size for hens were measured from 5 to 17 weeks.qRT-PCR was performed to analyze the hypothalalmic expression changes of c-Myc/LIN28B/let-7a pathways.The results showed that：1) The ovary and oviduct developed slowly and the follicles kept primitive in early period of development in Wenchang chicken.This period would last about 12 weeks.Then，the gonad tissues developed explosively，the ovary weight and oviduct length of 13 weeks increased 125% and 120% respectively compared to that of 12 weeks，and accompanied by emergence of pre-hierarchical follicles.Based on these，the crucial “timing” for transition from juvenile to puberty onset for Wenchang hens was determined as age of 13 weeks.2) qRT-PCR test revealed the relative expression levels of c-Myc and LIN28B genes in hypothalamus decreased significantly (P<0.05，P<0.01) at the transition timing of puberty onset in Wenchang chicken.The lower level of c-Myc could last to the age of first laying，but that of LIN28B recovered to higher level before the age of first laying.Accompanied with these changes was the significant increase of let-7a (P<0.01)，which could kept higher until the age of first laying.These results indicated that c-Myc→LIN28B┤let-7a regulating pathway was involved in chicken puberty onset.

Key words: Wenchang chicken, puberty onset, hypothalamus, gene expression

中图分类号:

S831.2

韩威，苏一军，朱云芬，李国辉，张会永，殷建玫，王克华，邹剑敏. 文昌鸡性成熟启动前后下丘脑c-Myc/LIN28B/let-7a通路表达规律分析[J]. 畜牧兽医学报, 2016, 47(4): 709-715.

HAN Wei，SU Yi-jun，ZHU Yun-fen，LI Guo-hui，ZHANG Hui-yong，YIN Jian-mei，WANG Ke-hua，ZOU Jian-min. Analysis on Changes of Hypothalamic c-Myc/LIN28B/let-7a Pathway Transcription during Puberty Onset in Wenchang Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 709-715.

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