传染性支气管炎病毒S1和N基因遗传变异频率的分析

doi:10.11843/j.issn.0366-6964.2015.06.015

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (6): 989-997.doi: 10.11843/j.issn.0366-6964.2015.06.015

传染性支气管炎病毒S1和N基因遗传变异频率的分析

盛晓丹^1，2#，徐怀英^1#，刘霞²，黄迪海²，曲新泽²，秦卓明^1，2*

（1.山东省农业科学院家禽研究所，济南 250100；2.山东省健牧生物药业有限公司，济南 250100）

收稿日期:2014-09-24 出版日期:2015-06-23 发布日期:2015-06-19
通讯作者: 秦卓明，E-mail：qinzm1997@163.com
作者简介:盛晓丹（1985-），女，山东潍坊人，助理研究员，主要从事动物传染病防控研究，E-mail：sxd1985wl@163.com；徐怀英（1976-），女，山东冠县人，副研究员，主要从事动物传染病和免疫学研究，E-mail：hyingxu@163.com。二人对本文同等贡献
基金资助:
国家自然科学基金（31372332）；科技部农转资金（SQ2013ECC600059）；山东省科技攻关重点支持项目（2009GG10009006）；济南市成果转化支持项目(2012CG92)

Genetic Variation Frequency of S1 and N Gene of Infectious Bronchitis Virus

SHENG Xiao-dan^1，2# ，XU Huai-ying^1# ，LIU Xia²，HUANG Di-hai²，QU Xin-ze²，QIN Zhuo-ming^1，2*

（1.Institute of Poultry Science，Shandong Academy of Agricultural Science，Jinan 250100，China；2.Shandong Jianmu Biological Pharmaceutical Co.，Ltd.，Jinan 250100，China）

Received:2014-09-24 Online:2015-06-23 Published:2015-06-19

摘要/Abstract

摘要：

旨在探讨中国北方地区传染性支气管炎病毒（IBV）的流行和分子变异，进而探讨IBV的遗传演变规律。利用生物信息学方法对IBV纤突蛋白（S1）和核蛋白（N）编码基因进行分析比较。结果显示：2012—2013年发生在山东、天津等北方地区的IBV流行株高度同源，其S1基因核苷酸（氨基酸）相似性为94.0%~100%（93.1%~100%），N基因为98.4%~99.1%（98.1%~99.8%），而与经典疫苗株H₁₂₀相似性均较低，且至少在S1基因的3个高变区产生了较多的点突变、缺失和插入，且变异集中在第53—150和250—290位，以高变区HVR I居多，GM/13在 62—65位缺失4个氨基酸。进一步的分析表明：IBV流行株S1基因与1998年在我国北方流行的A2株核苷酸相似性最高，为94.9%~97.2%，年核苷酸（nt）变异频率平均为2.4 nt×10^-3；而N基因则与LX4株相似性最高，为93.0%~93.5%，年变异频率平均为5.1 nt×10^-3。IBV流行株可能是A2-like和LX4-like毒株的重组，且N基因的变异频率明显高于S1基因。

关键词: IBV, S1基因, N基因, 遗传变异频率

Abstract:

This study was undertaken to investigate the prevalence and molecular variation of infectious bronchitis virus in the northern area of China.Thirteen infectious bronchitis viruses (IBV) strains obtained from commercial chickens in northern China between 2012 and 2013 were isolated and identified by the sequencing of the entire S1 gene.The variants were distinguished by phylogenetic analysis and alignment in comparison with the vaccination-challenge tests that were performed using heterogeneous strains.The results showed that both the S1 and N gene of the prevailing IBV shared higher homologies.Furthermore，S1 gene shared nucleotide (amino acid) homology of 94.0%-100% (93.1%-100%)，and N gene had nucleotide (amino acid) 98.4%-99.1% homology (98.1%-99.8%)，receptively.Compared with the classical vaccine strain，there were at least three hyper-variable regions were found in S1 gene，and point mutations，deletions and insertions of IBV strains were also occurred at the genomic level，which were concentrated in the 53-150，and 250-290.Four amino acids were found missing in GM/13 at 62-65.Further analysis showed that the IBV epidemic strains shared highest homology （94.9%-97.2%） with A2 strains and the nucleotide (nt) mutation frequency averaged 2.4 nt×10^-3/year in view of S1 gene；while，the N gene was the LX4 strains of the homologous highest (93.0%-93.5%)，the average annual variation in frequency 5.1 nt×10^-3/year.Those findings suggest that the recent IBV epidemic strains may be recombinant of A2 and LX4 type strains，and N gene mutation frequency is greater than of the S1 gene.

Key words: infectious bronchitis virus, S1 gene, N gene, mutation frequency

中图分类号:

S852.659.6

盛晓丹，徐怀英，刘霞，黄迪海，曲新泽，秦卓明. 传染性支气管炎病毒S1和N基因遗传变异频率的分析[J]. 畜牧兽医学报, 2015, 46(6): 989-997.

SHENG Xiao-dan，XU Huai-ying，LIU Xia，HUANG Di-hai，QU Xin-ze，QIN Zhuo-ming. Genetic Variation Frequency of S1 and N Gene of Infectious Bronchitis Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 989-997.

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传染性支气管炎病毒S1和N基因遗传变异频率的分析

Genetic Variation Frequency of S1 and N Gene of Infectious Bronchitis Virus

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