基于棉籽粕源发酵饲料的鸡血浆代谢组学研究

doi:10.11843/j.issn.0366-6964.2013.05.010

摘要/Abstract

摘要：

旨在将基于液相色谱-质谱（LC-MS）的代谢组学分析平台用于研究不同棉籽粕源发酵饲料对鸡血浆代谢物的影响。本研究用LC-MS采集对照组（CG）、假丝酵母组（CT）、酿酒酵母组（SC）和复合发酵组（CF）4组鸡血浆谱图，通过软件的峰识别和峰匹配等步骤得到峰表，采用偏最小二乘法-判别分析（PLS-DA）方法对样品进行分型，根据模型的变量重要性投影（VIP>1.5）和显著性差异检验结果(P<0.05)等筛选差异代谢物。结果表明：与CG相比，添加发酵饲料后鸡血浆代谢物存在显著差异，较CG组均极显著上调(P<0.01)。发生变化的主要化合物CT组为磷脂酰胆碱(PC)、磷脂酰乙醇胺(PE)、胆固醇酯（CE）、鞘磷脂（SM）、甘油二酯（DG）和甘油三酯（TG），SC组为PC、CE、SM、DG和TG，CF组为PC、PE和DG。饲粮中添加棉籽粕源微生物发酵饲料后，对鸡血浆代谢物产生了显著的影响，主要表现在脂类代谢物的增加，其中共同差异代谢物为PC和DG。

关键词: 液相色谱-质谱联用, 代谢组学, 发酵饲料, 鸡血浆

Abstract:

The objective of this study was to investigate the effect of fermented feed from cottonseed meal mixed substrate on chickens plasma metabolites via metabolomic technology based on high performance liquid chromatography-mass spectromentry (LC-MS). The data of plasma metabolites profile collected by LC-MS, which included four groups of control group (CG), Candida tropicalis group (CT), Saccharomyces cerevisiae group (SC) and compound fermentation group (CF), were transformed and the peak characterization was obtained by the commercial software automatically. The total difference of metabolites among CG, CT, SC and CF were found through partial least square-discriminant analysis (PLS-DA), and the differential metabolites were selected according to the value of variable importance in the projection（VIP>1.5）and P (P<0.05). The results showed that significant difference of plasma metabolites were found after adding the fermented feed to diets and the relative contents of these metabolites were all increased significantly (P<0.01) compared with the control group. These metabolites were mainly phosphatidylcholine (PC), phophatidylethanolamine (PE), cholesterol ester (CE), sphingomyelin (SM), diacylglycerol (DG) and triacylglycerol (TG) in CT; PC, CE, SM, DG, TG in SC; and PC, PE, DG in CF. The influence of microorganism fermented feed from cottonseed meal mixed substrate on chicken plasma metabolites mainly was revealed in the significant increase of lipid metabolites. The PC and DG were common difference metabolites in all treatment groups.

Key words: liquid chromatographic-mass spectrometry(LC-MS), metabolomics, fermented feed, chicken plasma

中图分类号:

S831.4

聂存喜，张文举，闫理东，蒋粒薪，马贵军. 基于棉籽粕源发酵饲料的鸡血浆代谢组学研究[J]. 畜牧兽医学报, 2013, 44(5): 737-744.

NIE Cun-xi, ZHANG Wen-ju, YAN Li-dong, JIANG Li-xin, MA Gui-jun. A Metabolomics Study on Chicken Plasma Based on Fermented Feed from Cottonseed Meal Mixed Substrate[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(5): 737-744.

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