畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (7): 1533-1539.doi: 10.11843/j.issn.0366-6964.2018.07.024

• 研究简报 • 上一篇    下一篇

基于代谢组学技术的鹿血与鹿茸血鉴别研究

张然然, 王磊, 李洋, 徐佳萍, 邢秀梅*   

  1. 中国农业科学院特产研究所 特种经济动物分子生物学重点实验室, 长春 130112
  • 收稿日期:2017-12-01 出版日期:2018-07-23 发布日期:2018-07-23
  • 通讯作者: 邢秀梅,研究员,E-mail:xingxiumei2004@126.com
  • 作者简介:张然然(1990-),女,河北深州人,助理研究员,硕士,主要从事特种经济动物种质资源保护与遗传育种研究,E-mail:heavenranran@163.com
  • 基金资助:

    特种动物遗传资源创新团队(CAAS-ASTIP-201X-ISAPS)

Distinguishing for the Deer Blood and Antler Blood by Metabolomics Approach

ZHANG Ran-ran, WANG Lei, LI Yang, XU Jia-ping, XING Xiu-mei*   

  1. Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China
  • Received:2017-12-01 Online:2018-07-23 Published:2018-07-23

摘要:

旨在研究鹿血与鹿茸血之间的小分子代谢物组分差异,为鹿血、鹿茸血相关产品的深度开发提供理论基础。采用超高液相色谱-四级杆-飞行时间质谱(UPLC-QTOF-MS)技术,结合多元统计分析方法(PCA、OPLS-DA)对鹿血与鹿茸血的整体代谢物组分进行比较,并进行定量分析。多元统计分析显示,鹿血与鹿茸血在X变量上区分明显,表明两者在小分子代谢物层面上是有着明显的区别。根据代谢物的峰值共筛选出28种显著差异表达代谢物,其中脂类、核苷酸及天然抗氧化剂-麦角硫因在鹿茸血中含量较高。鹿血与鹿茸血的代谢组学分析能够为鹿血相关产品的开发提供理论基础。

Abstract:

The purpose of this experiment was to study the differences of the metabolite components between deer blood and antler blood, and to provide a theoretical basis for the further development of related products. The metabolites were analyzed and quantified by UPLC-QTOF-MS combined with multivariate statistical analysis(PCA, OPLS-DA). Multivariate statistical analysis showed that the deer blood and antler blood were clearly distinguished in the direction of the variable X, indicating a significant difference in the small metabolite composition. Twenty-eight kinds of significantly differentially expressed metabolites were screened. The contents of lipids, nucleotides and ergothioneine-a natural antioxidant, were higher in antler blood. Metabolomics analysis of deer blood and antler blood can provide theoretical basis for the development of deer blood related products.

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