单色光对肉鸡体质量和小肠免疫应激相关因子IL-6与TNF-α表达的影响

畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (9): 1479-1482.

单色光对肉鸡体质量和小肠免疫应激相关因子IL-6与TNF-α表达的影响

胡易，姜楠，陈耀星，王子旭^*，曹静，董玉兰，白鹤

（中国农业大学动物医学院，北京 100193）

收稿日期:2012-06-20 出版日期:2012-09-25 发布日期:2012-10-26
通讯作者: 王子旭，高级实验师，主要从事黏膜免疫学研究，E-mail: zxwang2007@163.com
作者简介:胡易（1992-），男，安徽人，大学生，主要从事黏膜免疫学研究，E-mail: huyivet@126.com
基金资助:
国家大学生创新计划（201210019061）；北京市大学生创新计划；大学生科研训练计划（URP）；国家自然科学基金（31172277;31272516）；博士点基金(20100008110022)

Effect of Monochromatic Light on Body Weight and Expression of IL-6 and TNF-α #br# in Small Intestine of Broilers

HU Yi, JIANG Nan, CHEN Yao-xing, WANG Zi-xu^*, CAO Jing, DONG Yu-lan, BAI He

(College of Veterinary Medicine, China Agricultural University, Beijing 100193，China)

Received:2012-06-20 Online:2012-09-25 Published:2012-10-26

摘要/Abstract

摘要：

本试验旨在通过检测在单色光下饲养的肉鸡的体质量和空肠中细胞因子白细胞介素-6（IL-6）与肿瘤坏死因子（TNF-α）表达量的改变，探讨单色光对肉鸡生长发育和肠道免疫应激水平的影响。选用256只刚出壳的雄性AA肉鸡，随机分成4组，分别在蓝(480 nm)、绿(560 nm)、红(660 nm)、白(400～700 nm)4种光色下饲养。各组饲养管理制度相同，光照的强度为15 lx，光照时间为23 h。肉鸡42日龄时，称重后取空肠，用ELISA测定IL-6和TNF-α的表达量。试验结果显示与白光组肉鸡相比，蓝光组肉鸡的体质量高15.2%，而空肠IL-6和TNF-α的表达量分别降低了46.8%和33.4%（P＜0.05），绿光组、红光组、白光组3组间差异均不显著（P＞0.05）。结果表明蓝光能促进肉鸡生长和缓解光刺激诱发的小肠免疫应激。

关键词: 单色光, 免疫应激, 细胞因子, 小肠, 肉鸡

Abstract:

The aim of this study was to discover the effect of monochromatic light on the body weight and expression of IL-6 and TNF-α in the jejunum in broilers, which are two indicators of the stress degree, and define the role that monochromatic light plays towards the growth and immune stress procedure of intestine. 256 AA male broilers were randomly divided into 4 groups and exposed to blue light (480 nm, BL), green light (560 nm, GL), red light (660 nm, GL) and white light (400-700 nm, WL) at 1 day old, respectively. All light sources were equalized on the light illumination of 15 lx and schedule was L∶D = 23 h∶ 1 h. At 42 days old, the broilers were weighed, and jejunums were removed to detect the expression of IL-6 and TNF-α by ELISA. The results were as follows: 1) The body weigh of the BL group was 15.2% larger that of the WL group. 2) The expression of IL-6 and TNF-α were significantly decreased by 46.8% and 33.4% in the BL group than that of WL group, respectively (P＜0.05). However, no significant differences were observed between GL, RL and WL groups. These results indicated that the blue light can improve the growth in broilers and attenuated light-induced immune stress in the small intestine.

Key words: monochromatic light, immune stress, cytokine, small intestine, broiler

中图分类号:

S852.1

胡易, 姜楠, 陈耀星, 王子旭, 曹静, 董玉兰, 白鹤. 单色光对肉鸡体质量和小肠免疫应激相关因子IL-6与TNF-α表达的影响[J]. 畜牧兽医学报, 2012, 43(9): 1479-1482.

HU Yi, JIANG Nan, CHEN Yao-Xing, WANG Zi-Xu, CAO Jing, DONG Yu-Lan, BAI He. Effect of Monochromatic Light on Body Weight and Expression of IL-6 and TNF-α #br# in Small Intestine of Broilers[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(9): 1479-1482.

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