单色光对孵化后期鸡胚骨骼肌抗氧化能力的影响

doi:10.11843/j.issn.0366-6964.2018.09.021

摘要/Abstract

摘要：

本文旨在研究单色光对肉鸡胚胎发育后期骨骼肌抗氧化功能的影响。选取225枚AA肉鸡受精蛋随机分为5组，分别置于白光、蓝光、绿光、红光和黑暗条件下孵化（n=45）。于15胚龄（E15）、18胚龄（E18）和21胚龄（E21）时分别取胸大肌和腓肠肌，检测抗氧化酶（SOD、GSH-Px、CAT）活性、总抗氧化能力（T-AOC水平）和丙二醛（MDA）含量。结果如下：①在E18与E21时，白光组抗氧化酶活性和T-AOC水平高于黑暗组（0.92%～32.79%），而MDA含量减少（1.75%～10.32%）；②与白光组相比，绿光极显著增强骨骼肌抗氧化酶活性和T-AOC水平（1.72%～51.29%，P<0.01），显著降低MDA含量（8.78%～18.26%，P<0.05）；蓝光组骨骼肌抗氧化酶活性和T-AOC水平提高（0.84%~39.48%），MDA含量降低（2.35%～11.20%），但升高与降低程度均不及绿光组；红光组则相反，降低骨骼肌抗氧化酶活性与T-AOC水平（2.29%～28.17%），使MDA含量增加（0.51%～18.08%），该作用在E18与E21时尤为明显（P<0.01）。孵化期间给予单色绿光照射可增强肉鸡胚胎发育后期骨骼肌抗氧化功能，而红光则起抑制作用。

Abstract:

To study the effect of monochromatic light on antioxidative capacity of skeletal muscle in broiler during late-embryonic stage, a total of 225 AA broiler fertilized eggs were randomly divided into 5 groups and were incubated under white light, blue light, green light, red light and dark conditions, respectively. The pectoralis major and gastrocnemius muscles were taken at the 15th, 18th and 21st day of embryonic age (abbreviated as E15, E18 and E21, respectively). The activity of antioxidant enzymes (SOD, GSH-Px and CAT), total antioxidant capacity (T-AOC) and the content of malondialdehyde (MDA) were measured. Results were as follows:① At E18 and E21, the antioxidant enzymes activity and T-AOC levels in the white light group were higher than those in the dark group (0.92%-32.79%), but the MDA content was decreased (1.75%-10.32%); ② Compared with the white light group, especially at E18 and E21, the anti-oxidative enzyme activity and T-AOC levels in the green light group were significantly increased (1.72%-51.29%, P<0.01) and the MDA content was significantly decreased (8.78%-18.26%, P<0.05). The anti-oxidative enzyme activity and T-AOC levels under the blue light were increased (0.84%-39.48%) and the MDA content was decreased (2.35%-11.20%). However, the degrees of increase and decrease under the blue light were lower than that of the green light group. In contrast, the red light reduced the anti-oxidative enzyme activity and T-AOC levels (2.29%-28.17%) and increased the MDA content (0.51%-18.08%). Our results suggeste that the monochromatic green light illumination can enhance the antioxidation function of skeletal muscle in the late-embryonic stage of broiler, but monochromatic red light has inhibitory effect.

中图分类号:

S831.1

李婕, 杨秀娟, 曹静, 董玉兰, 陈耀星, 王子旭. 单色光对孵化后期鸡胚骨骼肌抗氧化能力的影响[J]. 畜牧兽医学报, 2018, 49(9): 1998-2004.

LI Jie, YANG Xiu-juan, CAO Jing, DONG Yu-lan, CHEN Yao-xing, WANG Zi-xu. Effect of Monochromatic Light on Antioxidative Capacity of Skeletal Muscle in Broiler during Late-Embryonic Stage[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 1998-2004.

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