利用微卫星DNA标记分析巴布亚新几内亚三个地方鸡群体的遗传多样性及亲缘关系

畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (7): 797-803.

利用微卫星DNA标记分析巴布亚新几内亚三个地方鸡群体的遗传多样性及亲缘关系

曾盛诚^1,2, Gariba Danbaro³, William Nano³, Sammy Doka³,Tau Gerega³, Olivier Hanotte⁴, 韩建林^2,4*

1. 中国农业科学院北京畜牧兽医研究所农业部畜禽遗传资源与利用重点开放实验室，北京100193；2. 中国农业科学院国际家畜研究所畜禽牧草遗传资源联合实验室，北京 100193；3. 巴布亚新几内亚理工大学农学院，巴布亚新几内亚411；4. 国际家畜研究所，肯尼亚内罗毕 00100

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-07-20 发布日期:2010-07-20
通讯作者: 韩建林

Genetic Diversity and Relationship of Three Indigenous Chicken Populationsfrom Papua New Guinea Using Microsatellite Analysis

ZENG Sheng-cheng^{1, 2}, Gariba Danbaro³, William Nano³,Sammy Doka³, Tau Gerega³, Olivier Hanotte⁴, HAN Jian-lin^{2, 4}*

1. Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture,Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;2. CAASILRI Joint Laboratory on Livestock and Forage Genetic Resources, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 3. Department of Agriculture, University of Technology, PMB, Lae 411, Papua New Guinea；4. InternationalLivestock Research Institute, P.O. Box 30709, Nairobi 00100, Kenya

Received:1900-01-01 Revised:1900-01-01 Online:2010-07-20 Published:2010-07-20

摘要/Abstract

摘要： 为了解巴布亚新几内亚地方鸡群体的遗传多样性和亲缘关系，本研究利用20个微卫星DNA遗传标记，对3个群体、96个个体的基因型进行了测定，估算了群体内的杂合度和近交系数（FIS）以及群体间的遗传分化系数（FST）等多样性参数；根据Nei氏标准遗传距离，构建群体水平的NJ树和个体水平上的无根UPGMA树。结果表明，3个群体内的平均期望（基因）杂合度介于0.665 9至0.680 6，平均FIS介于0.020至0.286，总群体水平上的遗传变异仅占5.5%；3个群体相互之间的遗传分化均达到显著水平，在个体水平的无根UPGMA树上，来源同一群体的个体会优先聚为一类。这说明巴布亚新几内亚的3个地方鸡群体的遗传多样性比较丰富，群体间的遗传分化显著，应该分别加以保护和利用。

关键词: 巴布亚新几内亚, 地方鸡, 微卫星, 遗传多样性

Abstract: To assess the genetic diversity within indigenous chicken populations from Papua New Guinea (PNG) and the relationship among them, 20 microsatellite DNA markers were used to genotype 96 samples of three PNG chicken populations. The heterozygosity and inbreeding coefficient (F_IS) within the populations and genetic differentiation coefficient (F_ST) among the populations were estimated among other diversity parameters. NJ tree at population level and un-rooted UPGMA trees at individual level using Nei′s standard genetic distances were reconstructed to examine the genetic relationships among the populations. The estimates of average expected (gene) heterozygosity and F_IS within the three populations ranged between 0.665 9 and 0.680 6, and between 0.020 and 0.286, respectively. The genetic differentiation among populations was 5.5% of total genetic variability. Majority of the individuals clustered into different groups in the unrooted UPGMA tree following their origins of sampling. The three Papua New Guinean indigenous chicken populations had a high level of genetic diversity within the populations and significant genetic differentiations among the populations, which called for specific attention on these unique genetic resources in the conservation and utilization programs.

Key words: Papua New Guinea, indigenous chicken, microsatellite, genetic diversity

曾盛诚;Gariba Danbaro;William Nano;Sammy Doka;Tau Gerega;Olivier Hanotte;韩建林;. 利用微卫星DNA标记分析巴布亚新几内亚三个地方鸡群体的遗传多样性及亲缘关系[J]. 畜牧兽医学报, 2010, 41(7): 797-803.

ZENG Sheng-cheng;;Gariba Danbaro;William Nano;Sammy Doka;Tau Gerega;Olivier Hanotte;HAN Jian-lin;. Genetic Diversity and Relationship of Three Indigenous Chicken Populationsfrom Papua New Guinea Using Microsatellite Analysis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(7): 797-803.

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