基于线粒体12S基因全序列分析我国绵羊痒螨(兔亚种)的遗传多样性

doi:10.11843/j.issn.0366-6964.2018.11.019

摘要/Abstract

摘要：

为了探讨我国绵羊痒螨（兔亚种）的遗传变异情况及群体遗传结构，采用PCR测序技术测定我国5个地区（华北、华东、华中、西北、西南）绵羊痒螨（兔亚种）线粒体12S基因的全序列，并进行遗传多样性分析。结果共获得89条序列，长度均为657 bp，核苷酸变异位点76个，单倍型50个（H1~H50），单倍型多样性（Hd）与核苷酸多样性（π）分别为0.931 05和0.005 59。进一步分析发现5个地理种群遗传分化程度较弱（F_st=0.042 322），基因交流非常频繁（Nm=5.657 372）。中性检验结果均为负值（Tajima's D=-0.928 31，Fu's Fs=-7.066 71），并且差异性不显著（P<0.05）。构建的NJ树和单倍型网络图均显示绵羊痒螨（兔亚种）5个地理种群的50个单倍型散在分布于不同的支系内，未形成明显的地理分布格局。我国绵羊痒螨（兔亚种）具有较高的遗传多样性，且有一定的遗传分化，基因交流频繁，但未形成以兔品种、温度带和地域来源划分的种群遗传结构。

Abstract:

To explore the genetic diversity and genetic structure of Psoroptes ovis var. cuniculi, the complete sequence of mitochondrial 12S gene of P. ovis var. cuniculi isolates from 5 geographical regions (Huabei, Huadong, Huazhong, Xibei, and Xinan) in China, were obtained by PCR sequencing techniques, and evaluated the genetic diversity of P. ovis population. Eighty-nine sequences of mitochondrial 12S were obtained. The multiple alignments of the full 12S gene from 89 isolates were 657 bp. These sequences contained 76 variable sites and were classified into 50 haplotypes (H1-H50). The values of haplotypes diversitiy (Hd) and nucleotide diversity (π) were 0.931 05 and 0.005 59 respectively. There were low level of genetic differentiation, but high level of gene flow (F_st=0.042 322, Nm=5.657 372) among the 5 geographical population. In P. ovis population, the average values of Tajima's D and Fu's Fs were negative (Tajima's D=-0.928 31, Fu's Fs=-7.066 71) and there was no significant difference (P<0.05). NJ phylogenetic tree and haplotype network were both revealed that 50 haplotypes from 5 different geographical regions dispersed across different clades without associating with the geographical locations. The results revealed that there was a high level of genetic diversity, little population genetic differentiation and high gene flow in the P. ovis var. cuniculi population of China, but population structure has not formed according to rabbits breed, temperature zones and geography origin.

中图分类号:

S852.746

李燕方, 古江, 袁媛, 谢跃, 赖为民, 杨光友, 古小彬. 基于线粒体12S基因全序列分析我国绵羊痒螨(兔亚种)的遗传多样性[J]. 畜牧兽医学报, 2018, 49(11): 2468-2476.

LI Yan-fang, GU Jiang, YUAN Yuan, XIE Yue, LAI Wei-min, YANG Guang-you, GU Xiao-bin. Genetic Diversity of Psoroptes ovis var. cuniculi Determined by the Full-length of Mitochondrial 12S Gene in China[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2468-2476.

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