基于线粒体16S rRNA全序列分析我国绵羊痒螨兔亚种的遗传多样性

doi:10.11843/j.issn.0366-6964.2018.05.016

摘要/Abstract

摘要：

为了探讨我国绵羊痒螨兔亚种的遗传变异特征和种群结构，利用PCR技术对我国5个地理区域（华北、华东、华中、西南、西北）的83个绵羊痒螨兔亚种株的线粒体16S rRNA全序列进行扩增和遗传多样性分析。结果显示，83个样品的16S rRNA全序列长度均为1 025 bp，共存在83个变异位点和52个单倍型；样品所代表的5个地理区域种群间的F_st值为-0.037 59~0.587 63，基因流值为-6.900 7~31.117 62；进一步用NJ树和单倍型网络图分析显示，52个单倍型构成2个大的分支，但却呈现出杂乱的分布格局，即地理区域、温度带和兔的品种特征性种群结构不显著。我国绵羊痒螨兔亚种的遗传多样性较高，遗传分化不明显，尚未形成地理种群结构。

Abstract:

In order to evaluate the genetic variation and population structure of Psoroptes ovis var. cuniculi, the full-length of the mitochondrial 16S rRNA of 83 isolates of Psoroptes ovis var. cuniculi from 5 geographic regions in China (including North, East, central, southwest, northwest zones) were amplified using the PCR technology, sequenced and then genetically analyzed. The results showed that the full 16S rRNA gene from 83 Psoroptes ovis var.cuniculi isolates were 1 025 bp in length, and that there were a total of 83 polymorphic sites and 52 haplotypes; the values of F_st and the gene flow between 5 geographical populations were -0.037 59-0.587 63 and -6.900 7-31.117 62, respectively; NJ tree and haplotype network analysis further showed these 52 haplotypes formed two branches with a messy distribution, and no population cluster patterns according to the geographical area, temperature zone and rabbit breeds were observed. These results indicate that Psoroptes ovis var.cuniculi population in China appeared a high genetic diversity and differentiation, but the geographical population structure has not formed.

中图分类号:

S852.746

古江, 李燕芳, 赵习彬, 谢跃, 赖为民, 杨光友, 古小彬. 基于线粒体16S rRNA全序列分析我国绵羊痒螨兔亚种的遗传多样性[J]. 畜牧兽医学报, 2018, 49(5): 1012-1019.

GU Jiang, LI Yan-fang, ZHAO Xi-bin, XIE Yue, LAI Wei-min, YANG Guang-you, GU Xiao-bin. Genetic Diversity of Psoroptes ovis var. cuniculi in China, Basing on the Complete Sequence of Mitochondrial 16S rRNA[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(5): 1012-1019.

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