一株新城疫重组强毒的分离鉴定与分子特性分析

畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (11): 1217-1223.

一株新城疫重组强毒的分离鉴定与分子特性分析

谭雷涛;秦卓明;马保臣;徐怀英;崔治中

1. 山东省农业科学院家禽研究所，济南 250100；2. 山东农业大学动物科技学院，泰安 271018

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-11-25 发布日期:2007-11-25

Molecular Characterization and Identification of A Velogenic Newcastle Disease Virus Isolate

1. Institute of Poultry Science,Shandong Academy of Agricultural Sciences, Jinan 250100,China;2. College of Animal Science and Technology,Shandong Agricultural University, Taian 271018,China

Received:1900-01-01 Revised:1900-01-01 Online:2007-11-25 Published:2007-11-25

摘要/Abstract

摘要： 从患病蛋鸡中分离出1株新城疫病毒（NDV）SRZ/03，经蚀斑纯化后接种40日龄SPF鸡可诱发典型ND病变。蚀斑纯化前、后，MDT分别为55.2和51 h，ICPI为1.7和2.0，IVPI为1.91和2.79，表明属强毒。对其F和HN基因分别克隆测序，进行F基因分型。结果表明SRZ/03属基因Ⅱ型，F蛋白氨基酸裂解位点的序列为112R-R-Q-K-R-F117，与强毒基序完全相同。结合GenBank中发表的其他NDV参考株序列，对F和HN基因推导氨基酸序列进行比较。SRZ/03株与基因Ⅱ型LaSota/46、B1/47、Bingham/87和Texas/48的同源性分别为93.1％、93.0％、94.4％和94.4％；与基因Ⅶ型Taiwan/95、SGM/01和SKY/03的同源性分别为92.2％、92.8％和92.8％；与基因Ⅲ型F48E9/48的同源性为91.2％。然而，SRZ/03株F蛋白前210个氨基酸与基因Ⅱ型LaSota/46和Texas/48的同源性较高，达96.7％和98.1％，后343个氨基酸则与Taiwan/95、SGM/01等毒株的同源性较高，达95.7％～97.4％，推测该株为重组株。此外，SRZ/03株与Taiwan/95、SGM/01等毒株的HN氨基酸同源性高达95.8％～97.6％，显著高于与LaSota/46、B1/47、Bingham/87、Texas/48和F48E9/48的87.2％～89.2％。

关键词: 新城疫病毒, 毒力, F基因, HN基因, 重组株

Abstract: A Newcastle disease virus (NDV) field strain named SRZ/03 was isolated from a layer flock with typical symptoms and lesions and identified by the HI test, and cloned by plaquepurification of three times. SRZ/03 was determined as a virulent strain with MDT of 55.2h and 51h , ICPI of 1.7 and 2.0, IVPI of 1.91 and 2.79 respectively before and after plaque-purification. Analysis of F gene indicated that SRZ/03 belonged to genotype Ⅱ, and the amino acid sequence deduced from the F gene at the cleavage site was 112R-R-Q-K-R-F117 which was the same as the velogenic virus, and the F protein amino acid sequence had 93.1％, 93.0％, 94.4％ and 94.4％ homologies with that belong to published genotype Ⅱvaccine strains LaSota/46, B1/47, virulent strain Bingham/87 and Texas/48, and very similar homologies of 92.2％, 92.8％ ,92.8％ or 91.2％ were found when comparing with Taiwan/95, SGM/01, SKY/03 and F48E9/48 that belong to published genotypes Ⅶ and Ⅲ. However, the former 210 amino acids of F protein of SRZ/03 shared 96.7％ and 98.1％ homology with that of LaSota/46 and Texas/48， respectively, and the highest homology of the latter 343 amino acids of F protein was 95.7％ and 97.4％ compared with Taiwan/95 and SGM/01, which indicated that SRZ/03 may be a recombinant strain. Furthermore, when HN genes were compared, SRZ/03 demonstrated higher homologies of 95.8％-97.6％ with the known velogenic strains such as Taiwan/95 or SGM/01 , but lower homologies of 87.2％-89.3％ with LaSota/46, B1/47, Bingham/87 and Texas/48.

Key words: Newcastle disease virus, virulence, F gene, HN gene, recombinant strain

谭雷涛;秦卓明;马保臣;徐怀英;崔治中. 一株新城疫重组强毒的分离鉴定与分子特性分析[J]. 畜牧兽医学报, 2007, 38(11): 1217-1223.

Molecular Characterization and Identification of A Velogenic Newcastle Disease Virus Isolate. Molecular Characterization and Identification of A Velogenic Newcastle Disease Virus Isolate[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2007, 38(11): 1217-1223.

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