畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (8): 1781-1788.doi: 10.11843/j.issn.0366-6964.2018.08.024

• 研究简报 • 上一篇    下一篇

4株新出现的Ⅰ类新城疫病毒F基因的变异分析

魏润宇1,2,4, 罗瑶瑶2,3, 吕艳2, 王静静2, 郑东霞2, 赵云玲2, 刘文博1,4*, 刘华雷2*, 王志亮2*   

  1. 1. 扬州大学兽医学院, 扬州 225009;
    2. 中国动物卫生与流行病中心, 青岛 266032;
    3. 青岛农业大学, 青岛 266109;
    4. 江苏高校动物重要疫病与人畜共患病防控协同创新中心, 扬州 225009
  • 收稿日期:2017-11-30 出版日期:2018-08-23 发布日期:2018-08-23
  • 通讯作者: 刘文博,E-mail:damoxiongying18@qq.com;刘华雷,E-mail:hualeiliuwy@163.com;王志亮,E-mail:zlwang111@163.com
  • 作者简介:魏润宇(1992-),女,山东淄博人,硕士生,主要从事预防兽医学研究,E-mail:1449623577@qq.com
  • 基金资助:

    国家重点研发计划(2016YFD0500800);公益性行业(农业)科研专项(201303033);江苏高校优势学科建设工程资助项目(PAPD)

Distribution and Molecular Characterization of 4 New Class Ⅰ Newcastle Disease Viruses Isolated from China

WEI Run-yu1,2,4, LUO Yao-yao2,3, LÜ Yan2, WANG Jing-jing2, ZHENG Dong-xia2, ZHAO Yun-ling2, LIU Wen-bo1,4*, LIU Hua-lei2*, WANG Zhi-liang2*   

  1. 1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;
    2. China Animal Health and Epidemiology Center, Qingdao 266032, China;
    3. Qingdao Agricultural University, Qingdao 266109, China;
    4. A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, Yangzhou 225009, China
  • Received:2017-11-30 Online:2018-08-23 Published:2018-08-23

摘要:

为了分析我国新出现的Ⅰ类新城疫病毒的分布和分子特性,对2016年分离到的4株Ⅰ类新城疫病毒进行F基因序列分析和遗传进化分析。结果显示:4株Ⅰ类新城疫病毒F基因编码长度均为1 662 nt,共编码553个氨基酸,分离株之间氨基酸相似性为97.9%~100%。4株分离株F蛋白裂解位点的氨基酸组成均为112ERQERL117,符合新城疫病毒弱毒株的分子特征。F基因高变区(47-420 nt)遗传进化分析结果显示这4株病毒为国内新出现的基因型,利用F基因编码区全长序列构建系统进化树显示4株病毒属于基因1c亚型,与北美分离株高度同源,而与国内基因1c亚型分离株(Duck/Guangxi/1261/2015)相似性仅为95.5%~97%。监测数据表明这种新出现的Ⅰ类新城疫病毒具有在国内进一步流行和扩散的风险。

Abstract:

In order to understand the distribution and molecular characteristics of new emerged class Ⅰ Newcastle disease virus in China. We carried out the F gene sequence analysis and phylogenetic analysis of 4 viruses isolated in 2016. Results were as follows:The length of complete F gene of 4 NDV isolates were 1 662 nt, encoding 553 amino acids, and the amino acid identity among all isolates was 97.9%-100%. The amino acid motif at F protein cleavage site of the 4 isolates was 112ERQERL117, which was consistent with that of lentogenic Newcastle disease virus. The phylogenetic analysis of F gene (47-420 nt) showed that all 4 isolates were newly emerging NDVs in China. Phylogenetic tree constructed by the complete F genes showed 4 Class Ⅰ viruses belonged to sub-genotype 1c, which showed a high level of similarity with isolates obtained from North America, and had the identity of 95.5%-97% with the sub-genotype 1c virus (Duck/Guangxi/1261/2015) isolated in China. Our results indicated that the new emerged class Ⅰ NDVs had the ability to circulate and spread in China.

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