高铜对大鼠肾细胞炎性因子和细胞增殖的影响

doi:10.11843/j.issn.0366-6964.2020.11.023

摘要/Abstract

摘要： 旨在探讨铜中毒对大鼠肾组织炎性因子的表达和细胞增殖功能的影响。本研究选用32只20日龄健康大鼠随机分为4组，每组8只，其中，对照组日粮的铜（Cu）浓度为15 mg·kg^-1；高铜Ⅰ组日粮Cu浓度为30 mg·kg^-1；高铜Ⅱ组日粮Cu浓度为60 mg·kg^-1；高铜Ⅲ组日粮Cu浓度为120 mg· kg^-1。连续饲喂6个月，检测肾组织Ki-67、PCNA、IL-1β、IL-2、IL-6、IL-18、NF-κB、TNF-α的mRNA及其蛋白的表达。随着日粮中铜含量增高，肾组织中Ki-67、PCNA mRNA及其蛋白表达量显著降低（P<0.05）。炎性因子IL-1β、IL-2、IL-6、IL-18、NF-κB、TNF-α mRNA的表达水平显著升高（P<0.05），IL-1β蛋白的表达水平呈剂量依赖性上升，IL-6和IL-18蛋白表达水平先上升，后下降。结果表明，日粮铜含量高于30 mg·kg^-1时，将不同程度地抑制大鼠肾组织的细胞增殖，促进炎性因子的表达，造成炎性损伤。

关键词: 铜, 肾, 细胞增殖, 炎性因子, 大鼠

Abstract: The purpose of this research was to investigate the effect of copper poisoning on the expression of inflammatory factors and cell proliferation in renal tissues of rat. In this study, 32 healthy rats aged 20 days were selected and randomly divided into 4 groups, 8 in each group. The rats were fed as follows: The copper concentration in the control group was 15 mg·kg^-1; high-copper group I: 30 mg·kg^-1; high-copper group Ⅱ: 60 mg·kg^-1; high-copper group Ⅲ: 120 mg·kg^-1. After continuously feeding for 6 months, the mRNA and their proteins expression of Ki-67, PCNA, IL-1β, IL-2, IL-6, IL-18, NF-κB, TNF-α in renal tissues were detected. The results demonstrated that the relative expressions of Ki-67，PCNA mRNA and proteins in renal tissues were significantly decreased with the increase of copper content in feed (P<0.05). Meanwhile, the mRNA expression levels of inflammatory factors including IL-1β, IL-2, IL-6, IL-18, NF-κB, TNF-α in renal tissues were significantly increased (P<0.05). Besides, the relative expression of IL-1β protein was up-regulated in a dose dependent manner, and the relative expression levels of IL-6 and IL-18 proteins increased first and then decreased. In summary, the finding suggested that high copper stimulation inhibited the cell proliferation of rat kidney tissue, promoted the expression of inflammatory factors, and caused inflammatory damage.

Key words: copper, renal, cell proliferation, inflammatory factors, rat

中图分类号:

S856.9

于晴晴, 钟高龙, 万方, 宁芷君, 伍少峰, 蒋璇璇, 唐兆新, 何颖, 胡莲美. 高铜对大鼠肾细胞炎性因子和细胞增殖的影响[J]. 畜牧兽医学报, 2020, 51(11): 2849-2857.

YU Qingqing, ZHONG Gaolong, WAN Fang, NING Zhijun, WU Shaofeng, JIANG Xuanxuan, TANG Zhaoxin, HE Ying, HU Lianmei. Effects of High Copper on Inflammatory Factors and Cell Proliferation in Rat Kidney Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(11): 2849-2857.

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