雌二醇对雌性家兔十二指肠肌电活动的作用研究

doi:10.11843/j.issn.0366-6964.2018.10.023

摘要/Abstract

摘要：

拟阐明雌激素调节十二指肠运动的作用规律，并筛选和建立其浓度与十二指肠肌电活动监测指标的数学模型。通过构建试验家兔的卵巢摘除动物模型和电极埋置动物模型，肌注不同剂量的苯甲酸雌二醇，采用BL-420F生物机能试验系统分别记录各试验组家兔十二指肠肌电活动变化，并对结果进行统计分析，结合对各监测指标的函数拟合精度分析选择并构建苯甲酸雌二醇与十二指肠肌电活动监测指标最优的数学模型。结果表明，十二指肠肌电的振幅、频率与肌电活动指数均随苯甲酸雌二醇剂量的增加呈现出一种先升高后降低的变化趋势。通过对各监测指标进行分析、筛选，构建了雌二醇剂量与肌电活动指数关系的函数模型，对该函数进行分析后可知，肌电活动指数的变化范围为1.50~5.23 mV·min^-1，并在雌二醇剂量为0.098 mg·kg^-1时肌电活动指数达到最大值5.23 mV·min^-1。此外，以雌二醇剂量0.063、0.098、0.133 mg·kg^-1为分界点，可将肌电活动指数随雌二醇剂量增加而变化的趋势分为指数型增加、对数型增加、指数型减小以及对数型减小四个区间段。上述结果表明雌二醇对十二指肠肌电活动呈现明显浓度依赖性双重调节作用，并且这种调节作用是在十二指肠本身具备一定水平的肌电活动的基础上进行的，即雌激素对十二指肠肌电活动的调节作用是间接的。此外十二指肠的肌电活动在不同浓度范围雌激素的作用下表现不同的变化趋势，呈现出明显的区间效应。本试验的研究结果为阐明雌激素在体内调节十二指肠运动的调节方式和雌激素类药物的研发和应用提供了一定理论依据和参考。

Abstract:

The objective of this study was to clarify the regulation of estrogen on the duodenal myoelectric motility and to screen and establish the mathematical model between the monitoring index of monitoring duodenal myoelectric activity and the concertration of estrogen. The ovariectomized and electrode embedded animal models of experimental rabbits were constructed and the different doses of estradiol benzoate were injected intramuscularly. The changes of myoelectric activity in duodenum of rabbits in each group were recorded by BL-420F biological function test system and the results were statistically analyzed. The optimal mathematical model of monitoring index between duodenal electromyography and estradiol benzoate was selected and constructed according to the function fitting accuracy analysis of each monitoring index. The results showed that the amplitude, frequency and the index of myoelectric activity of duodenal myoelectricity were all increased first, and then decreased with the increase of estradiol benzoate dose. The functional model of the relationship between the dosage of estradiol and the index of myoelectric activity was established through the analysis and screening of each monitoring index. The analysis results of this function showed that the index of myoelectric activity was changed on the range from 1.50 to 5.23 mV·min^-1, and the maximum value reached 5.23 mV·min^-1 when the dose of estradiol reached 0.098 mg·kg^-1. In addition, the trend of the index of myoelectric activity with the increase of estradiol dose could be divided into four intervals containing exponential increase, logarithmic increase, exponential decrease and logarithmic decrease with the estradiol dosage of 0.063, 0.098 and 0.133 mg·kg^-1 as the cutting point. The above results suggest that estradiol has a concentration-dependent dual regulatory effect on duodenal myoelectric activity, and this regulation is based on a certain level of myoelectric activity in the duodenum. The regulation of estrogen on duodenal myoelectric activity is indirect. In addition, the myoelectric activity of duodenum showed different tendency under the action of estrogen in different concentration and presented obvious interval effect. The results of this study provide a theoretical basis and reference for elucidating the regulation mode of estrogen in the regulation of duodenal movement in vivo and the development and application of estrogenic drugs.

中图分类号:

S852.2

杜宜楠, 郭雅茹, 赵建帅, 张金, 辛先萌, 马艺嘉, 何雪旻, 徐永平. 雌二醇对雌性家兔十二指肠肌电活动的作用研究[J]. 畜牧兽医学报, 2018, 49(10): 2268-2275.

DU Yi-nan, GUO Ya-ru, ZHAO Jian-shuai, ZHANG Jin, XIN Xian-meng, MA Yi-jia, HE Xue-min, XU Yong-ping. The Effect of Estrogen on the Regulation of Duodenal Myoelectric Activity of Female Rabbit[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(10): 2268-2275.

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