产乳酸菌素屎肠球菌B13对断奶仔猪生长性能、养分消化率、血清免疫指标及粪便微生物菌群的影响

doi:10.11843/j.issn.0366-6964.2017.10.013

摘要/Abstract

摘要：

旨在探讨高产乳酸菌素屎肠球菌B₁₃在饲料中添加对断奶仔猪生产性能、养分消化率、血清免疫指标、粪便微生物菌群的影响。本试验选用28日龄生长状况良好的“杜×长×大”三元杂交仔猪（初重（7.89±1.37）kg）56头，以体重为区组，分配到4个处理组中：A组（抗生素组）、B组（无抗组）、C组（复合菌组）和D组（试验菌组：屎肠球菌B₁₃），试验周期42 d。结果表明：1）C、D两组仔猪的全期平均日增重较B组均明显提高（P<0.05），钙的表观消化率较B组显著提高（P<0.05）；D组血清中IgG抗体水平较B组提高19.72%（P<0.05）。2）DGGE结果显示，添加屎肠球菌（D组）对粪便中细菌多样性有影响。3）RT-PCR结果显示，添加益生菌（C、D组）后仔猪粪便中乳酸菌及屎肠球菌数量较B组显著增加（P<0.05），并且大肠杆菌数量具有下降趋势（P=0.07）。综上表明，在本试验条件下，饲粮中添加屎肠球菌B₁₃能提高仔猪生产性能、粗蛋白及钙的消化率，增强仔猪免疫力；同时它还能够维持仔猪消化道菌群稳态，遏制致病微生物的生长。

Abstract:

This study was conducted to evaluate the effects of dietary Enterococcus faecium B₁₃ on growth performance, nutrient digestibility, serum immune parameters and fecal microbiota in weaned piglets. A total of 56 healthy crossbred “Duroc×Landrace× Yorkshire” weaned piglets at 28 days of age with an average body weight of (7.89 ± 1.37) kg were allotted to 4 treatments: group A (antibiotic group), group B (without antibiotic group), group C (compound bacteria group), group D (test group: Enterococcus faecium B₁₃), according to the complete randomized block design. The experiment lasted for 42 d. The result showed that: 1) The ADG and the apparent digestibility of Ca were improved in group C and D compared with group B(P<0.05). The level of IgG in group D was increased by 19.72%(P<0.05) compared with group B.2) Denaturing gradient gel electrophoresis (DGGE) showed that the application of E. faecium B₁₃ changed the bacterial community in fecal. 3) Real-time PCR results showed that, compared with group B, the number of Lactobacillus and E. faecium were increased (P<0.05) and the number of Escherichia coli had decreasing tendency (P = 0.07) in group C and D. Therefore, under the condition of this experiment, the dietary of E. faecium B₁₃ supplementation can improve the growth performance, digestibility of crude protein and calcium and enhance immune responses in weaned piglets. It can maintain the stability of digestive tract microflora of piglets and prevent the growth of pathogenic microorganisms.

中图分类号:

S828.4

丁爽, 郭春华, 张正帆, 柏雪, 魏婕, 张明, 罗璠. 产乳酸菌素屎肠球菌B₁₃对断奶仔猪生长性能、养分消化率、血清免疫指标及粪便微生物菌群的影响[J]. 畜牧兽医学报, 2017, 48(10): 1902-1911.

DING Shuang, GUO Chun-hua, ZHANG Zheng-fan, BAI Xue, WEI Jie, ZHANG Ming, LUO Fan. Effects of Enterococcus faecium B₁₃ Yielding Bacteriocin on Growth Performance, Nutrient Digestibility, Serum Immune Indexes and Fecal Microbiota of Weaned Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(10): 1902-1911.

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产乳酸菌素屎肠球菌B₁₃对断奶仔猪生长性能、养分消化率、血清免疫指标及粪便微生物菌群的影响

Effects of Enterococcus faecium B₁₃ Yielding Bacteriocin on Growth Performance, Nutrient Digestibility, Serum Immune Indexes and Fecal Microbiota of Weaned Piglets

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