中国荷斯坦牛初产日龄遗传评估及全基因组关联分析

doi:10.11843/j.issn.0366-6964.2015.03.004

摘要/Abstract

摘要：

基于高通量SNPs测序的全基因组关联分析为奶牛繁殖性状相关基因的探索提供了契机。本研究基于课题组前期中国荷斯坦牛基因组测序芯片的结果，通过DMU (v 6.0)采用AI-REML结合EM算法的动物模型对北京地区2001-2011年10年间19 111头中国荷斯坦母牛初产日龄记录进行遗传参数估计，并应用PLINK (v 1.07)将大群估计的2 172头中国荷斯坦母牛初产日龄性状的育种值（EBV）作为表型值，进行基于无关群体设计的全基因组关联分析。通过全基因组水平的Bonferroni校正，共检测到1 700个SNPs与初产日龄显著相关。选取P值达到10^-15的43个SNPs进行初步分析，其中12个位于X染色体上。显著SNPs上下游200 kb范围内存在KLHL4、TRAM1、TRAM2、ZNF438、MATK等繁殖障碍疾病相关基因。7号染色体1 Mb（20.42~21.52 Mb）区域内多个SNPs位点与初产日龄显著相关。这些基因和区域可以作为影响初产日龄性状的重要候选基因和区域，为揭示奶牛繁殖性状的分子遗传基础积累了素材。

Abstract:

Genome-wide association studies (GWAS) based on high throughput SNPs genotyping technology provided basis for exploring genes associated with fertility traits in Chinese Holsteins.Data of 19 111 heifers with calving records over a period of 10 years (2001-2011) were collected from Sanyuanlühe Dairy Cattle Breeding Center.Firstly，by AI-REML combined EM algorithm，DMU software (v 6.0) was used to estimate genetic parameters for age at first calving (AFC) based on animal model.Then GWAS was performed by the software PLINK (v 1.07) on the basis of the EBVs of calving traits of 2 172 Chinese Holstein heifers to identify genes affecting the fertility traits using the Illumina BovineSNPs50 BeadChip.The results showed that 1 700 SNPs was significantly associated with AFC at genome-wide level.Preliminarily，43 significant SNPs with P-valves smaller than 10^-15 were chosen to do the bioinformatics analyses.Among the 43 SNPs，12 were located on X chromosome，while some were within 1 Mb（20.42-21.52 Mb）on SSC7.In particular，the candidate genes of KLHL4，TRAM1，TRAM2，ZNF438 and MATK were related to some reproductive disturbances or diseases.The current study explored some novel SNPs as well as potential candidate genes associated with heifer calving traits，which can be used to unravel the genetic foundation of fertility traits in dairy cattle.

中图分类号:

S823.2

刘澳星，郭刚，王雅春，郭翔羽，张旭，刘林，石万海，李锡智，苏国生，张勤. 中国荷斯坦牛初产日龄遗传评估及全基因组关联分析[J]. 畜牧兽医学报, 2015, 46(3): 373-381.

LIU Ao-xing， GUO Gang， WANG Ya-chun， GUO Xiang-yu， ZHANG Xu，LIU Lin，SHI Wan-hai， LI Xi-zhi，SU Guo-sheng，ZHANG Qin. Genetic Analysis and Genome Wide Association Studies for Age at first Calving in Chinese Holsteins[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(3): 373-381.

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https://www.xmsyxb.com/CN/Y2015/V46/I3/373

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