高、低脂鸡脂肪组织ATGL基因的表达及其激素调控的差异分析

doi:10.11843/j.issn.0366-6964.2013.12.003

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (12): 1874-1881.doi: 10.11843/j.issn.0366-6964.2013.12.003

高、低脂鸡脂肪组织ATGL基因的表达及其激素调控的差异分析

史铭欣^1,2,3，荣恩光^1,2,3，周纬男^1,2,3，史洪岩^1,2,3，李辉^1,2,3，王宁^1,2,3*

(1.农业部鸡遗传育种重点实验室，哈尔滨 150030； 2.黑龙江省普通高等学校动物遗传育种与繁殖重点实验室，哈尔滨 150030； 3.东北农业大学动物科学技术学院，哈尔滨 150030）

收稿日期:2013-03-15 出版日期:2013-12-23 发布日期:2013-12-23
通讯作者: 王宁，教授，E-mail: wayane123@aliyan.com
作者简介:史铭欣(1988-)，女，山东莱阳人，硕士生，主要从事家禽遗传育种研究，E-mail: haoyan.4869@163.com
基金资助:
国家重点基础研究发展计划（973课题）(2009CB941604)；黑龙江省教育厅家禽遗传育种科技创新团队( 2010td02)

Adipose Tissue Expression and Hormonal Regulation of ATGL Gene in the Fat and Lean Broiler Lines

SHI Ming-xin^1,2,3, RONG En-guang^1,2,3, ZHOU Wei-nan^1,2,3, SHI Hong-yan^1,2,3, LI Hui^1,2,3, WANG Ning^1,2,3*

(1.Key Laboratory of Chicken Genetics and Breeding of Ministry of Agriculture, Harbin 150030, China;2.Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, China; 3.College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China)

Received:2013-03-15 Online:2013-12-23 Published:2013-12-23

摘要/Abstract

摘要：

为研究脂肪甘油三酯脂肪酶（ATGL）在鸡脂肪沉积中的作用，并分析肾上腺素和胰高血糖素对高、低脂鸡脂肪组织和脂肪细胞ATGL基因表达的影响，本研究利用半定量RT-PCR分析鸡ATGL基因的组织表达谱；利用Real-time RT-PCR分析ATGL基因及其活化因子Comparative gene identification-58（CGI-58）基因在1～7周龄东北农业大学肉鸡高、低腹脂双向选择品系（NEAUHLF）第16世代鸡群脂肪组织中的表达差异，以及肾上腺素和胰高血糖素对高、低脂系肉鸡脂肪组织和脂肪细胞ATGL基因表达的影响。结果显示，ATGL基因在鸡腹部脂肪、皮下脂肪、嗉囊周围脂肪、肌胃周围脂肪、肾脏及肝脏中表达量较高；低脂系肉鸡腹部脂肪组织ATGL和CGI-58基因的表达总体均高于高脂系鸡，且均在第2周龄达到显著性差异（P＜0.05）；肾上腺素和胰高血糖素都能够上调高脂系鸡脂肪组织和脂肪细胞ATGL基因的表达，并促进机体的脂肪分解,暗示高脂系鸡对2种激素的刺激相对敏感。本研究结果为揭示高、低脂肉鸡脂肪性状差异的原因以及ATGL在鸡脂肪沉积中的作用奠定了基础。

Abstract:

This study aimed to investigate the function of adipose triglyceride lipase（ATGL）in chicken fat deposition, and to analyze the effects of adrenaline and glucagon on the gene expression of ATGL in adipose tissue and adipocytes of the fat and lean broilers. The tissue expression pattern of ATGL gene was analyzed by semi-quantitative RT-PCR (SqRT-PCR). The expression of ATGL gene and its activator Comparative gene identification 58 (CGI-58) gene in abdominal adipose tissue and adipocytes in the fat and lean broiler lines of the 16th generation of NEAUHLF at 1-7 week-old was analyzed by Real-time RT-PCR. The results showed that chicken ATGL gene was highly expressed in abdominal fat, subcutaneous fat, crop fat, kidney, liver and gizzard fat. On the whole, the mRNA expression levels of ATGL and CGI-58 genes were higher in the lean broiler lines than that in the fat broiler lines. In particular, the expression of ATGL and CGI-58 genes in the lean broiler lines was significantly higher than that in the fat broiler lines at 2 week-old (P＜0.05). Adrenaline and glucagon up-regulated the expression of ATGL gene in the abdominal adipose tissue and adipocytes of the fat broiler lines and promoted lipolysis, which indicated that the fat broiler lines were more sensitive to the both hormones than the lean broiler lines. This study not only laid the foundation for revealing the difference of the fat phenotype of fat and lean broiler lines, but also offered a basis for clarifying the function of ATGL in fat deposition in broiler.

中图分类号:

S831.2

史铭欣，荣恩光，周纬男，史洪岩，李辉，王宁. 高、低脂鸡脂肪组织ATGL基因的表达及其激素调控的差异分析[J]. 畜牧兽医学报, 2013, 44(12): 1874-1881.

SHI Ming-xin, RONG En-guang, ZHOU Wei-nan, SHI Hong-yan, LI Hui, WANG Ning. Adipose Tissue Expression and Hormonal Regulation of ATGL Gene in the Fat and Lean Broiler Lines[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(12): 1874-1881.

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参考文献

［1］VILLENA J A, ROY S, SARKADI-NAGY E, et al. Desnutrin, an adipocyte gene encoding a novel patatin domain-containing protein, is induced by fasting and glucocorticoids: ectopic expression of desnutrin increases triglyceride hydrolysis［J］. J Biol Chem, 2004, 279(45): 47066-47075.

［2］SERR J M. The regulation of adipose triglyceride lipase-mediated lipolysis in avian species: the role of comparative gene identification-58［D］. USA: The Ohio State University, 2011: 14-17.

［3］KIM J Y, TILLISON K, LEE J H, et al. The adipose tissue triglyceride lipase ATGL/PNPLA2 is downregulated by insulin and TNF-alpha in 3T3-L1 adipocytes and is a target for transactivation by PPARgamma［J］. Am J Physiol Endocrinol Metab, 2006, 291:E115-127.

［4］YANG X, LU X, LIU J. Identification of a novel splicing isoform of murine CGI-58［J］. FEBS Lett, 2010a, 584:903-910.

［5］GRUBER A, CORNACIU I, LASS A, et al. The N-terminal region of comparative gene identification-58 (CGI-58) is important for lipid droplet binding and activation of adipose triglyceride lipase［J］. J Biol Chem, 2010, 285:12289-12298.

［6］SCHWEIGER M, LASS A, ZIMMERMANN R, et al. Neutral lipid storage disease: genetic disorders caused by mutations in adipose triglyceride lipase/PNPLA2 or CGI-58/ABHD5［J］. Am J Physiol Endocrinol Metab, 2009, 297:289-296.

［7］MONTERO-MORAN G, CAVIGLIA J M, MCMAHON D, et al. CGI-58/ABHD5 is a coenzyme A-dependent lysophosphatidic acid acyltransferase［J］. J Lipid Res, 2010, 51(4): 709-719.

［8］YANG X, LU X, LOMBES M, et al. The G(0)/G(1) switch gene 2 regulates adipose lipolysis through association with adipose triglyceride lipase［J］. Cell Metab, 2010, 11(3): 194-205.

［9］LEE K, SHIN J, LATSHAW J D, et al. Cloning of adipose triglyceride lipase complementary deoxyribonucleic acid in poultry and expression of adipose triglyceride lipase during development of adipose in chickens［J］. Poult Sci, 2009, 88(3): 620-630.

［10］SAARELA J, JUNG G, HERMANN M, et al. The patatin-like lipase family in Gallus gallus［J］. BMC Genomics, 2008, 9: 281.

［11］NIE Q, FANG M, XIE L, et al. cDNA cloning, characterization, and variation analysis of chicken adipose triglyceride lipase (ATGL) gene［J］. Mol Cell Biochem, 2009, 320(1-2): 67-74.

［12］SERR J, SUH Y, OH S A, et al. Acute up-regulation of adipose triglyceride lipase and release of non-esterified fatty acids by dexamethasone in chicken adipose tissue［J］. Lipids, 2011, 46(9): 813-820.

［13］LANGSLOW D R, HALES C N. Lipolysis in chicken adipose tissue in vitro［J］. J Endocrinol, 1969, 43: 285-294.

［14］LANGSLOW D R. The action of gut glucagon-like immunoreactivity and other intestinal hormones on lipolysis in chicken adipocytes［J］. Horm Metab Res, 2009, 5(06): 428-432.

［15］WANG Y, MU Y, LI H, et al. Peroxisome proliferator-activated receptor-γ gene: A key regulator of adipocyte differentiation in chickens［J］. Poult Sci, 2008, 87(2): 226-232.

［16］ZHANG H, KUMAR S, BARNETT A, et al. Ceiling culture of mature human adipocytes: use in studies of adipocyte functions［J］. Endocrinol, 2000, 164(2): 119-128.

［17］HE J, JIANG H, TANSEY J T, et al. Calyculin and okadaic acid promote perilipin phosphorylation and increase lipolysis in primary rat adipocytes［J］. Biochim Biophys Acta, 2006, 1761(2):247-255.

［18］NIE Q, HU Y, XIE L, et al. Identification and characterization of adipose triglyceride lipase (ATGL) gene in birds［J］. Mol Biol Rep, 2010, 37(7): 3487-3493.

［19］LU L, WANG F, CHEN X, et al. cDNA cloning, expression and regulation analysis of goose adipose triglyceride lipase (ATGL) gene［J］. Can J Anim Sci, 2011, 91(3): 363-369.

［20］DEIULIIS J A, SHIN J, BAE D, et al. Developmental, hormonal, and nutritional regulation of porcine adipose triglyceride lipase (ATGL) ［J］. Lipids, 2008, 43(3): 215-225.

［21］ZIMMERMANN R, STRAUSS J G, HAEMMERLE G, et al. Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase［J］. Science, 2004, 306(5700): 1383-1386.

［22］LU X, LIU J, YANG X. Differential control of ATGL-mediated lipid droplet degradation by CGI-58 and G0S2［J］. Cell Cycle, 2010, 9(14): 2719-2725.

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高、低脂鸡脂肪组织ATGL基因的表达及其激素调控的差异分析

Adipose Tissue Expression and Hormonal Regulation of ATGL Gene in the Fat and Lean Broiler Lines

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