哺乳动物精子获能前后的理化变化及获能机制研究进展

doi:10.11843/j.issn.0366-6964.2013.12.002

摘要/Abstract

摘要：

综述了精子获能前后的运动轨迹和质膜微观结构的改变，及与之相伴的代谢类型、酶系活性和相关离子流动等一系列理化变化，并从胆固醇外流、活性氧产生、蛋白质磷酸化、膜电位极化等方面阐述了获能机理，同时指出，蛋白酪氨酸磷酸化是精子获能的关键。提出应用转录组学、蛋白组学结合生物信息学等技术，研究获能过程胆固醇转运体的作用方式、胞外Ca²⁺对获能的影响、与质膜胆固醇流失相伴的膜外Ca²⁺、HCO₃^-内流顺序及其关系、与信号传导相关蛋白的酪氨酸磷酸化等重要获能事件，将为深入研究精子获能机制提供一定的理论参考。

Abstract:

Before and after sperm capacitation, the motion trail and membrane microstructure were changed, and associated physiochemical change events of the metabolic types, enzyme activity and fluidity of related ions. In addition, we also reviewed the mechanism of capacitation from the cholesterol efflux, production of reactive oxygen species (ROX), protein tyrosine phosphorylation (PTP) and variation membrane potential. Meanwhile, the importance of PTP during sperm capacitation was pointed out in current review. We offered that the combined use of bioinformatics tools and proteomic/transcriptomics would be applicated on the study about cholesterol efflux from plasma membranes to acceptors mediated by the transporters, the effect of extracellular Ca²⁺ on capacitation, the inflow turn of extracellular Ca²⁺ and HCO₃^- accompanied with the cholesterol efflux and their relationship, as well as the PTP related to the signaling pathway, which could provide a theoretical guidance for further study on capacitation mechanism.

中图分类号:

S814.8

贺亚南，朱化彬，陈晓丽，郝海生，秦彤，赵学明，路永强，王栋. 哺乳动物精子获能前后的理化变化及获能机制研究进展[J]. 畜牧兽医学报, 2013, 44(12): 1867-1873.

HE Ya-nan, ZHU Hua-bin, CHEN Xiao-li, HAO Hai-sheng, QIN Tong, ZHAO Xue-ming, LU Yong-qiang, WANG Dong. Advance of Capacitation Mechanism and the Physiochemical Changes of Sperm before and after Capacitation of Mammalian[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(12): 1867-1873.

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参考文献

［1］RISOPATRON J, CATALAN S, MISKA W, et al. Effect of albumin and polyvinyl alcohol on the vitality, motility and acrosomal integrity of canine spermatozoa incubated in vitro［J］. Reprod Domest Anim, 2002, 37(6): 347-351.

［2］BEDFORD J M. Sperm capacitation and fertilization in mammals［J］. Biol Reprod Suppl, 1970, 2: 128-158.

［3］ZANEVELD L J, DE JONGE C J, ANDERSON R A, et al. Human sperm capacitation and the acrosome reaction［J］. Hum Reprod, 1991, 6(9): 1265-1274.

［4］HAN H L, MACK S R, DE JONGE C, et al. Inhibition of the human sperm acrosome reaction by a high molecular weight factor from human seminal plasma［J］. Fertil Steril, 1990, 54(6): 1177-1179.

［5］GILLAN L, MAXWELL W M, EVANS G. Preservation and evaluation of semen for artificial insemination［J］. Reprod Fertil Dev, 2004, 16(4): 447-454.

［6］陆金春, 张福泉, 黄字烽, 等. 精子获能的研究进展［J］. 中华男科学, 2002, 8(4): 296-298.

［7］ VISCONTI P E, KRAPF D, DE LA VEGA-BELTRAN J L, et al. Ion channels, phosphorylation and mammalian sperm capacitation［J］. Asian J Androl, 2011, 13(3): 395-405.

［8］CHEN X L, ZHU H B, WU C J, et al. Identification of differentially expressed proteins between bull X and Y spermatozoa［J］. J Proteomics, 2012, 77: 59-67.

［9］ZHAO C, GUO X J, SHI Z H, et al. Role of translation by mitochondrial-type ribosomes during sperm capacitation: an analysis based on a proteomic approach［J］. Proteomics, 2009, 9(5): 1385-1399.

［10］BAKER M A, REEVES G, HETHERINGTON L, et al. Analysis of proteomic changes associated with sperm capacitation through the combined use of IPG-strip pre-fractionation followed by RP chromatography LC-MS/MS analysis［J］. Proteomics, 2010, 10(3): 482-495.

［11］KOTA V, DHOPLE V M, SHIVAJI S. Tyrosine phosphoproteome of hamster spermatozoa: role of glycerol-3-phosphate dehydrogenase 2 in sperm capacitation［J］. Proteomics, 2009, 9(7): 1809-1826.

［12］FICARRO S, CHERTIHIN O, WESTBROOK V A, et al. Phosphoproteome analysis of capacitated human sperm. Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation［J］. J Biol Chem, 2003, 278(13): 11579-11589.

［13］BAILEY J L, TARDIF S, DUBE C, et al. Use of phosphoproteomics to study tyrosine kinase activity in capacitating boar sperm Kinase activity and capacitation［J］. Theriogenology, 2005, 63(2): 599-614.

［14］JAGAN MOHANARAO G, ATREJA S K. Identification of capacitation associated tyrosine phosphoproteins in buffalo (Bubalus bubalis) and cattle spermatozoa［J］. Anim Reprod Sci, 2011, 123(1-2): 40-47.

［15］VISCONTI P E, KOPF G S. Regulation of protein phosphorylation during sperm capacitation［J］. Biol Reprod, 1998, 59(1): 1-6.

［16］LEWIS B, AITKEN R J. Impact of epididymal maturation on the tyrosine phosphorylation patterns exhibited by rat spermatozoa［J］. Biol Reprod, 2001, 64 (5): 1545-1556.

［17］张媛媛, 胡启蒙, 王亮亮, 等. 哺乳动物精子磷酸化蛋白质组学研究进展［J］. 中国生物工程杂志, 2012, 32(4): 76-82.

［18］刘国艺, 李彦清, 倪江.牛精子体外获能后的超微结构变化［J］. 实验动物科学与管理, 2002, 19(1): 12-14.

［19］YANAGIMACHI R. In vitro capacitation of golden hamster spermatozoa by homologous and heterologous blood sera［J］. Biol Reprod, 1970, 3(2): 147-153.

［20］熊承良, 商学军, 刘继红. 人类精子学［M］. 武汉：湖北科学技术出版社, 2002: 73-74.

［21］邹思湘. 动物生物化学［M］. 北京：中国农业出版社, 2005:174-179.

［22］BREITBART H. Signaling pathways in sperm capacitation and acrosome reaction［J］. Cell Mol Biol, 2003, 49(3): 321-327.

［23］石其贤. 精子获能 (I)［J］. 浙江省医学科学院学报, 1980, 3(1): 61-65.

［24］FERNANDES A P, CURI G, FRANCA F G, et al. Nuclear changes and acrosome formation during spermiogenesis in Euchistus heros (Hemiptera: Pentatomidae)［J］. Tissue Cell, 2001, 33(3): 286-293.

［25］郑行, 朱化彬. 高级动物生殖生理［M］. 北京：中国农业科学院研究生院, 2009: 59-60.

［26］MACK S, BHATTACHARYYA A K, JOYCE C, et al. Acrosomal enzymes of human spermatozoa before and after in vitro capacitation［J］. Biol Reprod, 1983, 28(5): 1032-1042.

［27］吴承疆, 朱化彬, 王栋, 等. 牛冻、鲜精子差异蛋白的双向电泳和质谱鉴定的初步研究［J］. 中国畜牧兽医, 2010, 37(11): 100-104.

［28］ARCELAY E, SALICIONI A M, WERTHEIMER E, et al. Identification of proteins undergoing tyrosine phosphorylation during mouse sperm capacitation［J］. Int J Dev Biol, 2008, 52(5-6): 463-472.

［29］SCHACKMANN R W, SHAPIRO B M. A partial sequence of ionic changes associated with the acrosome reaction of Strongylocentrotus purpuratus［J］. Dev Biol, 1981, 81(1): 145-154.

［30］THUNDATHIL J C, ANZAR M, BUHR M M. Na⁺/K⁺ ATPase as a signaling molecule during bovine sperm capacitation［J］. Biol Reprod, 2006, 75 (3): 308-317.

［31］WERTHEIMER E. Involvement of ion fluxes and cAMP pathways in sperm capacitation［D］. UMass Amherst, 2010.

［32］LIU B, ZHANG W, WANG Z. Voltage-dependent anion channel in mammalian spermatozoa［J］. Biochem Biophys Res Commun, 2010, 397(4): 633-636.

［33］SAMPSON M J, DECKER W K, BEAUDET A L, et al. Immotile sperm and infertility in mice lacking mitochondrial voltage-dependent anion channel type 3［J］. J Biol Chem, 2001, 276(42): 39206-39212.

［34］PETROUNKINA A M, HARRISON R A, PETZO-LDT R, et al. Cyclical changes in sperm volume during in vitro incubation under capacitating conditions: a novel boar semen characteristic［J］. J Reprod Fertil, 2000, 118(2): 283-293.

［35］PURDY P H, GRAHAM J K. Effect of adding cholesterol to bull sperm membranes on sperm capacitation, the acrosome reaction, and fertility［J］. Biol Reprod, 2004, 71(2): 522-527.

［36］ABOU-HAILA A, TULSIANI D R P. Signal transduction pathways that regulate sperm capacitation and the acrosome reaction［J］. Arch Biochem Biophys, 2009, 485(1): 72-81.

［37］DEMARCO I A, ESPINOSA F, EDWARDS J, et al. Involvement of a Na⁺/HCO₃^- cotransporter in mouse sperm capacitation［J］. J Biol Chem, 2003, 278(9): 7001-7009.

［38］DE LAMIRANDE E, LAMOTHE G. Reactive oxygen-induced reactive oxygen formation during human sperm capacitation［J］. Free Radic Biol Med, 2009, 46 (4): 502-510.

［39］彭礼繁, 罗光彬, 李东全, 等. 牛精子体外获能的研究进展［J］. 中国草食动物, 2009, 1: 57-61.

［40］SHADAN S, JAMES P S, HOWES E A, et al. Cholesterol efflux alters lipid raft stability and distribution during capacitation of boar spermatozoa［J］. Biol Reprod, 2004, 71(1): 253-265.

［41］GALANTINO H L, ZENG W X, MEGEE S O, et al. Effects of 2-hydroxypropyl-beta-cyclodextrin and cholesterol on porcine sperm viability and capacitation status following cold shock or incubation［J］. Mol Reprod Dev, 2006, 73(5): 638-650.

［42］MORALES C R, NI X, SMITH C E, et al. ABCA17 mediates sterol efflux from mouse spermatozoa plasma membranes［J］. Histol Histopathol, 2012, 27(3): 317-328.

［43］WEIGEL N M. Kinases and protein phosphorylation as regulators of steroid hormone action［J］. Nuclear Recept Signal, 2007, 5: e005.

［44］KUMAR V, RANGARAJ N, SHIVAJI S. Activity of pyruvate dehydrogenase A (PDHA) in hamster spermatozoa correlates positively with hyperactivation and is associated with sperm capacitation［J］. Biol Reprod, 2006, 75(5): 767-777.

［45］周思畅, 倪崖, 石其贤. 获能期间精子蛋白的酪氨酸磷酸化［J］. 生命科学, 2006, 18(3): 285-289.

［46］JHA K N, SHIVAJI S. Protein serine and threonine phosphorylation, hyperactivation and acrosome reaction in vitro capacitated hamster spermatozoa［J］. Mol Reprod Dev, 2002, 63(1): 119-130.

［47］VISCONTI P E, GALANTINO-HOMER H, NING X, et al. Cholesterol efflux-mediated signal transduction in mammalian sperm. beta-cyclodextrins initiate transmembrane signaling leading to an increase in protein tyrosine phosphorylation and capacitation［J］. J Biol Chem, 1999, 274(5): 3235-3242.

［48］DA ROS V G, MUNUCE M J, COHEN D J, et al. Bicarbonate is required for migration of sperm epididymal protein DE (CRISP-1) to the equatorial segment and expression of rat sperm fusion ability［J］. Biol Reprod, 2004, 70(5): 1325-1332.

［49］HUANG J Y, WANG G L, KONG L J. Effects of Ca²⁺ and HCO₃^-on capacitation, hyperactivation and protein tyrosine phosphorylation in Guinea pig spermatozoa［J］. Asian-Austral J Anim, 2009, 22(2): 181-186.

［50］BAKER M A, HETHERINGTON L, ECROYD H, et al. Analysis of the mechanism by which calcium negatively regulates the tyrosine phosphorylation cascade associated with sperm capacitation［J］. J Cell Sci, 2004, 117(2): 211-222.

［51］李东全，罗光彬，张小华, 等. 哺乳动物精子获能的分子机制［J］. 动物医学进展, 2005, 8: 13-16.

［52］ZENG Y, CLARK E N, FLORMAN H M. Sperm membrane potential: hyperpolarization during capacitation regulates zona pellucida-dependent acrosomal secretion［J］. Dev Biol, 1995, 171(2): 554-563.

［53］龚伟. 促发精子获能的分子机制［J］. 中华男科学, 2003, 9(9): 693-696.

［54］张瑞芳, 曾海涛. 钙离子通道与精子运动功能［J］. 生命的化学, 2009, 29(6): 878-882.

［55］JAIN R K, JAIN A, KUMAR R, et al. Functional attenuation of human sperm by novel, non-surfactant spermicides: precise targeting of membrane physiology without affecting structure［J］. Hum Reprod, 2010, 25(5): 1165-1176.

［56］RIVLIN J, MENDEL J, RUBINSTEIN S, et al. Role of hydrogen peroxide in sperm capacitation and acrosome reaction［J］. Biol Reprod, 2004, 70(2): 518-522.

［57］DONA G, FIORE C, TIBALDI E, et al. Endogenous reactive oxygen species content and modulation of tyrosine phosphorylation during sperm capacitation［J］. Int J Androl, 2011, 34(5 Pt 1): 411-419.

［58］DE LAMIRANDE E, O’FLAHERTY C. Sperm activation: role of reactive oxygen species and kinases［J］. Biochim Biophys Acta, 2008, 1784(1): 106-115.

［59］CORMIER N, BAILEY J L. A differential mechanism is involved during heparin- and cryopreservation-induced capacitation of bovine spermatozoa［J］. Biol Reprod, 2003, 69(1): 177-185.

［60］PAVLOK A, KUBELKA M, PEKNICOVA J. The effect of various capacitation active compounds and capacitation time on the in vitro fertility and protein tyrosine phosphorylation profiles of bovine sperm［J］. Zygote, 2001, 9(1): 25-38.

［61］RODRIGUEZ P C, SATORRE M M, BECONI M T. Effect of two intracellular calcium modulators on sperm motility and heparin-induced capacitation in cryopreserved bovine spermatozoa［J］. Anim Reprod Sci, 2012, 131(3-4): 135-142.

［62］WILLIAMS A C, FORD W C. The role of glucose in supporting motility and capacitation in human spermatozoa［J］. J Androl, 2001, 22(4): 680-695.

［63］PARRISH J J, SUSKO-PARRISH J, WINER M A, et al. Capacitation of bovine sperm by heparin［J］. Biol Reprod, 1988, 38(5): 1171-1180.

［64］STOREY B T. Mammalian sperm metabolism: oxygen and sugar, friend and foe［J］. Int J Dev Biol, 2008, 52(5-6): 427-437.

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