A 型塞内卡病毒抗体检测胶体金免疫层析试纸条的制备及初步应用

doi:10.11843/j.issn.0366-6964.2025.08.045

Abstract

Abstract:

The aim of this study was to develop a rapid, accurate and easy-to-use colloidal gold assay for Senecavirus A (SVA) antibody diagnosis for Senecavirus infection. The recombinant protein of SVA VP2 was obtained by prokaryotic expression and purification, and the VP2 protein polyclonal antibody was prepared. The VP2 protein was coupled with colloidal gold to form a gold labeled antigen. Subsequently, VP2 protein and VP2 polyclonal antibody were coated on nitrocellulosic membrane (NC membrane) as test line (T-line) and control line (C-line), respectively. Optimizing the reaction system to prepare test strips. The results showed that the prepared test strips had no cross reactivity and good specificity when detecting foot-and-mouth disease virus (FMDV) serotypes O and A (FMDV-O, FMDV-A), porcine reproductive and respiratory syndrome virus (PRRSV), classical swine fever virus (CSFV), pseudorabies virus (PRV), African swine fever virus (ASFV) positive serum and healthy pig (SPF) serum; The sensitivity of SVA positive serum was 1 ∶64. The kappa value of the strip and virus neutralization test (VNT) was 0.88 in 150 swine clinical samples, indicating that the two methods were highly consistent. In summary, an SVA antibody test strip was successfully developed by this study, which can be operated in 10 to 15 minutes, and has the advantages of being rapid, accurate and simple, providing an effective tool for clinical qualitative detection and on-site diagnosis of Senecavirus disease.

Key words: Senecavirus antibodies, VP2 protein, colloidal gold immunochromatographic test strip

CLC Number:

S852.659.6

LI Shuaipeng, SHI Zhengwang, CHEN Jie, LUO Juncong, ZHU Yuqian, SHI Xintai, XI Tao, ZHANG Fan, HE Yindi, ZHENG Haixue, ZHANG Xiaoli, TIAN Hong. Preparation and Preliminary Application of Colloidal Gold Immunochromatographic Test Strip for Senecavirus A Antibody Detection[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(8): 4086-4094.

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方法 Method		病毒中和试验 Virus neutralization test
方法 Method		阳性 Positive	阴性 Negative	合计 Total
试纸条 Test strip	阳性 Positive	13	3	16
	阴性 Negative	0	134	134
	合计 Total	13	137	150
Kappa值 Kappa value	0.88

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Preparation and Preliminary Application of Colloidal Gold Immunochromatographic Test Strip for Senecavirus A Antibody Detection

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