河南省犬腺病毒Ⅱ型的流行情况调查及其分离鉴定

doi:10.11843/j.issn.0366-6964.2024.12.047

Abstract

Abstract:

In order to investigate the epidemic characteristics and variation of canine adenovirus type 2 (CAV-2) in Henan Province, a total of 790 dog fecal samples were randomly collected in eight regions of Henan Province from 2020 to 2021. The pathogen detection of CAV-2 was carried out by PCR method, and the genetic evolution analysis was carried out based on E3 gene. At the same time, MDCK cells were used to isolate the virus, and the isolated strain was further identified by negative staining transmission electron microscopy, cell sensitivity spectrum test, erythrocyte hemagglutination spectrum test and physical and chemical characteristics analysis. The results showed that the total positive rate of CAV-2 was 1.39% (11/790). Region, age and immunization status could significantly affect the positive rate of CAV-2 (P < 0.05). Among them, Zhoukou City (3/40, 7.50%) had the highest infection rate. Dogs within 6 months old and immunocompromised were susceptible, and the positive rates were 2.69% (8/297) and 1.91% (10/524), respectively. There was no significant relationship between season and sex of dogs and viral infection (P>0.05). Eleven positive samples were sequenced and 5 sequences were obtained. Based on the genetic evolution analysis of CAV-2 E3 gene, 4 sequences were located in the same branch as the domestic CAV-2 HB1 strain, and 1 sequence was located in the same branch as the European and American strains. The positive samples were identified by plaque purification and virus identification. It was confirmed that the isolated virus was CAV-2, named CAV2-HN21. This study enriched the molecular epidemiological data of CAV-2 and successfully isolated a CAV-2 strain, which provided a theoretical basis for the follow-up study of CAV-2 prevention and control measures.

Key words: Henan province, CAV-2, prevalence survey, virus isolation and identification

CLC Number:

S855.3

GUO Jinjie, ZHENG Liping, ZUO Shoujun, SONG Pengtao, LI Liangliang, CHEN Guizhen, CHANG Zhihai, DAI Hongyu, LIU Fang, DONG Haiju. Epidemiological Investigation and Isolation of Canine Adenovirus Type Ⅱ in Henan Province[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(12): 5866-5872.

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References 21

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因素 Factor		阳性数/总样品数 Positive/Total	阳性率/% (95%CI) Positive rate (95%CI)	OR (95%CI)	P值 P value
地区 Region	郑州 Zhengzhou	4/150	2.67 (0.06~5.27)	1.00	0.03
	开封 Kaifeng	1/79	1.27 (-1.25~3.79)	2.137 (0.24~19.45)
	许昌 Xuchang	0/20	-	0.97 (0.95~1.00)
	焦作 Jiaozuo	1/150	0.67 (-0.65~1.98)	4.08 (0.45~36.96)
	安阳 Anyang	1/104	0.96 (-0.95~2.87)	2.82 (0.31~25.62)
	南阳 Nanyang	1/135	0.74 (-0.72~2.21)	3.67 (0.41~33.26)
	漯河 Luohe	0/112	-	0.97 (0.95~1.00)
	周口 Zhoukou	3/40	7.50 (-1.03~16.03)	0.34 (0.72~1.58)
性别 Sexuality	公犬 Male dog	5/348	1.45 (0.18~2.69)	1.00	0.93
性别 Sexuality	母犬 Female dog	6/442	1.36 (0.27~2.44)	1.06 (0.32~3.50)	0.93
年龄 Age	≤6月龄 ≤6 months old	8/297	2.69 (0.84~4.55)	1.00	0.02
年龄 Age	>6月龄 >6 months old	3/493	0.61 (-0.08~1.30)	4.521 (1.19~17.18)	0.02
免疫 Immunity	未免疫 Pre-immune	6/421	1.43 (0.29~2.56)	1.00	0.04
	未完全免疫 Not fully immune	4/103	3.88 (0.09~7.68)	0.357 (0.10~1.29)
	完全免疫 Absolute immunity	1/266	0.38 (-0.36~1.12)	3.82 (0.46~31.93)
季节 Season	春季 Spring	4/236	1.69 (0.04~3.35)	1.00	0.52
	夏季 Summer	1/115	0.87 (-0.85~2.59)	1.97 (0.22~17.79)
	秋季 Fall	1/196	0.51 (-0.50~1.52)	3.36 (0.37~30.33)
	冬季 Winter	5/243	2.06 (0.26~3.86)	0.82 (0.22~3.09)

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Epidemiological Investigation and Isolation of Canine Adenovirus Type Ⅱ in Henan Province

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地区 Region	年龄 Age		免疫 Immunity			季节 Season
地区 Region	≤6月龄 ≤6 months old	>6月龄 >6 months old	未免疫 Pre-immune	未完全免疫 Not fully immune	完全免疫 Absolute immune	春季 Spring	夏季 Summer	秋季 Fall	冬季 Winter
郑州 Zhengzhou	53	97	50	37	63	31	61	17	41
开封 Kaifeng	18	61	33	20	26	34	19	26	0
许昌 Xuchang	9	11	20	0	0	0	20	0	0
焦作 Jiaozuo	52	98	83	12	55	29	0	121	0
安阳 Anyang	37	67	73	5	26	61	0	0	43
南阳 Nanyang	64	71	79	8	48	45	0	0	90
漯河 Luohe	49	63	68	10	34	23	0	32	57
周口 Zhoukou	15	25	15	11	14	13	15	0	12
总计 Total	297	493	421	103	266	236	115	196	243