热休克蛋白27对水泡性口炎病毒体外增殖的调控作用

doi:10.11843/j.issn.0366-6964.2024.06.025

Abstract

Abstract:

This study was conducted to investigate the effect and mechanism of heat shock protein 27 (HSP27) on the proliferation of vesicular stomatitis virus (VSV) in vitro and the retinoid acid-inducible gene-I-like receptor (RLR) signaling pathway mediated by VSV infection. Real-time quantitative PCR, Western blotting, Co-immunoprecipitation, TCID₅₀ assay, and immunofluorescence methods were employed to reveal the effects of VSV infection on the mRNA and protein expression of HSP27, and then the effects of overexpression and interference with HSP27 on the proliferation of VSV, and further explore the effects of HSP27 on the RLR signaling pathway mediated by VSV infection. Finally, the interaction and co-localization between HSP27 and the target molecules in RLR signaling pathway were analyzed. The results showed that VSV infection could increase the gene transcription and protein expression of HSP27, both stable and transient overexpression of HSP27 significantly inhibited the proliferation of VSV in vitro. Interfering with HSP27 expression in host cells promoted the proliferation of VSV. Further studies showed that HSP27 enhanced both VSV and retinoic acid-inducible gene I protein (RIG-I) mediated IFN-β production and the protein expression of RIG-I, and that HSP27 interacted with RIG-I and co-localized in the cytoplasm. This study for the revealed the molecular mechanism by which HSP27 targets RIG-I to upregulate its expression, enhance the transduction of RLR signaling pathways, and then negatively regulate the proliferation of VSV in vitro, laying a foundation for further revealing the mechanism of host factor HSP27 in viral infection.

Key words: heat shock protein 27, vesicular stomatitis virus, RLR signaling pathway, RIG-I

CLC Number:

S852.659.5

Dianyu LI, Rongqian MO, Xu ZHAO, Ming GAO, Hongshan LI, Huisheng BAI, Ruixian MA, Xiangrong LI, Ruofei FENG. Regulation of Heat Shock Protein 27 on the Proliferation of Vesicular Stomatitis Virus in vitro[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(6): 2540-2549.

Figures/Tables 7

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References 24

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引物名称 Names	核苷酸序列(5′→3′) Nucleotide sequences
Homo-HSP27-qF	CTGACGGTCAAGACCAAGGATG
Homo-HSP27-qR	GTGTATTTCCGCGTGAAGCACC
Homo-GAPDH-qF	GTCTCCTCTGACTTCAACAGCG
Homo-GAPDH-qR	ACCACCCTGTTGCTGTAGCCAA
Homo-IFN-β-qF	TTGTTGAGAACCTCCTGGCT
Homo-IFN-β-qR	TGACTATGGTCCAGGCACAG

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Regulation of Heat Shock Protein 27 on the Proliferation of Vesicular Stomatitis Virus in vitro

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