检测兔出血症病毒抗体胶体金试纸条的研制及初步应用

doi:10.11843/j.issn.0366-6964.2018.03.019

Abstract

Abstract:

A colloidal gold test strip for rapid detection of RHDV antibody was developed based on colloidal gold immunochromatographic assay. The colloidal gold particles were labeled with recombinant RHDV VP60 protein. The anti-VP60 monoclonal antibody A3C and Staphylococcus aureus protein A (SPA) were respectively coated on the control line (C line) and test line (T line). The strip was optimized by detecting strong positive, positive, weak positive and negative serum, and its qualities and coincidence rate of HI test were verified. The results indicated that the T and C lines were the same color when the positive serum was detected. The T line was darker than the C line when the strong positive serum was detected. The T line was lighter than the C line when the weak positive serum was detected. Compared with HI test, the coincidence rates of strong positive, positive, weak positive, negative serum were 93.75%, 88.75%, 91.25%, 95.00%, respectively, while the total coincidence rate was 91.54%. The colloidal gold strip has specificity, sensitivity, repeatability and stability, and provide a tool for epidemiological survey and antibody level monitoring.

CLC Number:

WEI Hou-jun, FAN Zhi-yu, WANG Fang, SONG Yan-hua, HU Bo, QIU Ru-long, CHEN Meng-meng, XU Wei-zhong, XUE Jia-bin. Development and Preliminary Application of a Colloidal Gold Test Strip for Rabbit Hemorrhagic Disease Virus Antibody[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(3): 614-619.

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Development and Preliminary Application of a Colloidal Gold Test Strip for Rabbit Hemorrhagic Disease Virus Antibody

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