陕西部分地区山羊毕氏肠微孢子虫多位点基因分型研究

doi:10.11843/j.issn.0366-6964.2017.09.019

Abstract

Abstract:

To indicate the genetic variability of Enterocytozoon bieneusi in goats, the multi-locus sequence genotypes (MLGs) of 122 E. bieneusi isolates from different production categories were studied using the multi-locus sequence genotyping (MLST) technique based on micro-(MS1, MS3, MS7) and mini-satellite (MS4) loci. The results showed that, the respective amplification efficiencies in loci MS1, MS4 and MS7 were 27.9%(34/122), 18.0%(22/122), 50.8%(62/122), while no amplifications were detected in the locus MS3. The nucleotide sequences analysis indicated that, 16, 9 and 18 genotypes were found in all positive samples for loci MS1, MS4 and MS7, respectively, forming fourteen MLGs. Among them, the amplification efficiencies of three loci among 50 positive samples of cashmere goats were 10.0% (5/50), 14.0% (7/50) and 90.0% (45/50), respectively, belonging to three, three and ten genotypes, and five MLGs. The amplification efficiencies of 56 positive samples of dairy goats in three loci were 28.6% (16/56), 19.6% (11/56) and 8.9% (5/56), grouping into four, four, one genotypes, and seven different MLGs. The amplification efficiencies of three loci of 16 positive samples of black goats were 81.3% (13/16) and 25.0% (4/16), 75.0% (12/16), clustering into 9, 2, 7 genotypes, and two MLGs.

CLC Number:

S852.723

LIU Ting-li, PENG Xian-qi, SONG Jun-ke, HU Rui-si, WANG Sha-sha, PAN Guang-lin, ZHAO Guang-hui. Multi-locus Sequence Genotyping Study of Enterocytozoon bieneusi in Goats from Partial Areas of Shaanxi Province[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(9): 1730-1736.

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Multi-locus Sequence Genotyping Study of Enterocytozoon bieneusi in Goats from Partial Areas of Shaanxi Province

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