ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (11): 2069-2077.doi: 10.11843/j.issn.0366-6964.2015.11.021

Previous Articles     Next Articles

Study on the Preparation and Acute Toxicity of Tosufloxacin Nanoemulsion

GAO Xian1，OUYANG Wu-qing1* ，LI Meng-yun2，LIU Yue1，WANG Bo-zhen3，ZHENG Yin1，YANG Ming-qi1*   

  1. （1.College of Veterinary Medicine，Northwest A & F University，Yangling 712100，China；2.College of Animal Science and Veterinary，Henan University of Science and Technology，Luoyang 471003，China；3.College of Animal Science and Veterinary，Shihezi University，Shihezi 832003，China）
  • Received:2015-02-16 Online:2015-11-23 Published:2015-11-23

PDF (PC)

457

Abstract:

The purpose of this study was to prepare tosufloxacin nanoemulsion and to analyse its physical and chemical properties and acute toxicity.The oil phase，surfactant and solubilizer were selected according to the drug loading and stability of the nanoemulsion，the optimum formula was designed using pseudo-ternary phase diagrm，prepared tosufloxacin nanoemulsion.The configuration and particle size of tosufloxacin nanoemulsion were measured by TEM and LPSA.The drug content of tosufloxacin nanoemulsion was determined through High Performance Liquid Chromatography（HPLC）.The stability and shelf life were tested by stress testing，accelerated testing and long-term testing.Its acute toxicity was evaluated by gavage testing in mice.The results showed that the optimum formula of tosufloxacin nanoemulsion was composed of tosufloxacin（1.36%），origanum oil（3.24%），lactic acid（2.60%），RH-40（25.98%），and distilled water（66.82%）.The droplet shape of this nanoemulsion was spherical，with the average diameter of 16.16 nm，and its stability was good，the shelf life was 24 months.As the concentrations of tosufloxacin ranged from 3 to 100 μg•mL-1，the concentrations and peak areas showed good linear relationship；the average recovery rate and relative standard deviation of tosufloxacin nanoemulsion were 99.33%±1.24% and 1.25%，respectively；the precision of this nanoemulsion was well.The LD50 of tosufloxacin nanoemulsion was greater than 10 000 mg•kg-1，the MTD of this nanoemulsion was 39.72 g•kg-1.The tosufloxacin nanoemulsion was prepared successfully，and it will provide a theoretical basis for the further development of nanoscale antimicrobial agents.

CLC Number: