猪STARD3NL基因克隆和表达谱分析

doi:10.11843/j.issn.0366-6964.2015.09.026

Abstract

Abstract:

The aim of this study was to clone porcine STARD3NAL(STARD3 N-terminal like protein)，and analyze the structure of STARD3NAL，and investigate its expression in porcine different tissues.The porcine STARD3NAL gene was cloned by 5′，3′-RACE and RT-PCR，the protein structure of porcine STARD3NL was analyzed by bioinformatics methods.The expression patterns in different tissues were detected by real-time RT-PCR.The results showed that the porcine STARD3NL cDNA was 1 319 bp(GenBank accession No.：HQ634946) in length，which coded 235 amino acids and shared highly identity with those of other species.The phylogenetic tree analysis indicated the porcine STARD3NL was closely related to cattle and sheep，but distantly related to the zebrafish.The porcine STARD3NL protein contained conserved MENTAL domain and was hydrophobicity protein and there were 4 transmembrane helices in the STARD3NL protein.The secondary structure of STARD3NL protein showed that the STARD3NL protein fold for 60% into α-helix.This polypeptide contained no signal peptide in its amino acids，and was a nonsecretory protein.The residing probability of STARD3NL in the endoplasmic reticulum was 39.1%.By real-time PCR，mRNA expression of porcine STARD3NL were detected in all 14 tissues investigated，and STARD3NL mRNA expression levels in liver were the highest and in longissimus dorsi were the lowest.In addition，no significant difference of STARD3NL mRNA expression levels in lung tissues was detected between Laiwu pigs(LW) and YL pigs(Yorshire♂×Landrace♀) in infected circovirus group(P>0.05).These results will provide the data for further research of STARD3NL gene structures and function，breeding for disease resistance.

CLC Number:

S828
S813.3

XING Jin-yi，JIA Kun-hang，LI Yan-ping. Molecular Cloning and Expression Patterns Analysis of the Porcine STARD3 N-terminal like Protein(STARD3NL) Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1678-1685.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/ 10.11843/j.issn.0366-6964.2015.09.026

https://www.xmsyxb.com/EN/Y2015/V46/I9/1678

References

［1］ALPY F，WENDLING C，RIO M C，et al.MENTHO，a MLN64 Homologue devoid of the START domain［J］.J Biol Chem，2002，277(52)：50780-50787.
［2］ALPY F，TOMASETTO C.MLN64 and MENTHO，two mediators of endosomal cholesterol transport［J］.Biochem Soc Trans，2006，34（Pt 3）：343-345.
［3］ALPY F，LATCHUMANAN V K，KEDINGER V，et al.Functional characterization of the MENTAL domain［J］.J Biol Chem，2005，280(18)：17945-17952.
［4］ALPY F，TOMASETTO C.Give lipids a START：the StAR-related lipid transfer(START) domain in mammals［J］.J Cell Sci，2005，118（Pt 13）：2791-2801.
［5］ALPY F，ROUSSEAU A，SCHWAB Y，et al.STARD3 or STARD3NL and VAP form a novel molecular tether between late endosomes and the ER［J］.J Cell Sci，2013，126（Pt 23）：5500-5512.
［6］SIMONS K，IKONEN E.How cells handle cholesterol［J］.Science，2000，290（5497）：1721-1726.
［7］HÖLTTÄ-VUORI M，ALPY F，TANHUANPÄÄ K，et al.MLN64 is involved in actin-mediated dynamics of late endocytic organelles［J］.Mol Biol Cell，2005，16（8）：3873-3886.
［8］REITZ J，GEHRIG-BURGER K，STRAUSS III J F，et al.Cholesterol interaction with the related steroidogenic acute regulatory lipid-transfer(START) domains of StAR(STARD1) and MLN64(STARD3)［J］.FEBS J，2008，275(8)：1790-1802.
［9］NAKABAYASHI M，SHIBATA N，KOMORI H，et al.Structure of a conserved hypothetical protein，TTHA0849 from Thermus thermophilus HB8，at 2.4 a resolution：a putative member of the StAR-related lipid-transfer(START) domain superfamily［J］.Acta Crystallogr F，2005，61：1027-1031.
［10］ROMANOWSKI M J，SOCCIO R E，BRESLOW J L，et al.Crystal structure of the Mus musculus cholesterol-regulated START protein 4(StarD4) containing a StAR-related lipid transfer domain［J］.Proc Natl Acad Sci USA，2002，99(10)：6949-6954.
［11］TSUJISHITA Y，HURLEY J H.Structure and lipid transport mechanism of a StAR-related domain［J］. Nat Struct Biol，2000，7(5)：408-414.
［12］KISHIDA T，KOSTETSKII I，ZHANG Z，et al.Targeted mutation of the MLN64 START domain causes only modest alterations in cellular sterol metabolism［J］.J Biol Chem，2004，279(18)：19276-19285.
［13］ALPY F，BOULAY A，MOOG-LUTZ C，et al.Metastatic lymph node 64(MLN64)，a gene overexpressed in breast cancers，is regulated by Sp/KLF transcription factors［J］.Oncogene，2003，22(24)：3770-3780.
［14］BIÈCHE I，TOMASETTO C，RÉGNIER C H，et al.Two distinct amplified regions at 17q11-q21 involved in human primary breast cancer［J］.Cancer Res，1996，56(17)：3886-3890.
［15］KAURANIEMI P，BÄRLUND M，MONNI O，et al.New amplified and highly expressed genes discovered in the ERBB2 amplicon in breast cancer by cDNA microarrays［J］.Cancer Res，2001，61(22)：8235-8240.
［16］TOMASETTO C，RÉGNIER C，MOOG-LUTZ C，et al.Identification of four novel human genes amplified and overexpressed in breast carcinoma and localized to the q11-q21.3 region of chromosome 17［J］.Genomics，1995，28(3)：367-376.
［17］LI W F，HOU S X，YU B，et al.Genetics of osteoporosis：accelerating pace in gene identification and validation［J］.Hum Genet，2010，127(3)：249-285.

[1]	WANG Ji-ying, WANG Yan-ping, XU Yun-hua, WANG Cheng, LIN Hai-chao, HU Hong-mei, WU Ying, GUO Jian-feng. Analyses of Intramuscular Fat Content, Fatty Acid Composition and the Related Traits in Lulai Black Pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 585-594.
[2]	WANG Ping，HAO Wen-yan，CAO Li-hua，SHEN Kai-yuan，DAI Xiao-li，LI Hai-yan，LIANG Xian-wei，SHI De-shun，LI Xiang-ping. Cloning and Analysis of ssc-miR-148a Promoter [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 1969-1976.
[3]	DING Rong-rong，YANG Ming，QUAN Jian-ping,YANG Lin-xue，LIU Jing-shun，LIU Jia，GU Ting，LIU De-wu，CAI Geng-yuan，WU Zhen-fang，YANG Jie. A Comparative Study of Carcass and Meat Quality Traits between DLY and PDLY Pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(9): 1795-1805.
[4]	SUN Hao，WANG Zi-shuai，XU Yin-xue，TANG Zhong-lin. The Correlation between H19 Single Nucleotide Polymorphism and Pig Growth Characters and Its Spatio-temporal Expression [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 870-881.
[5]	HAN Li-qiang，GUO Yu-jie，LU Wei-fei，ZHANG Xin，CHU Bei-bei，WANG Jiang，YANG Guo-yu. Construction and Function Confirmation of MC4R Gene Eukaryotic Expression Vector with Different Mutations [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 679-685.
[6]	JI Jiu-xiu，FANG Shao-ming，ZHANG Zhen，ZENG Zhi-jun，MA Jun-wu，CHEN Cong-ying. Correlations of Blood Glucose and Lipids with Fat Deposition in Pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 686-692.
[7]	ZHANG Suo-yu，QIU Xiao-tian，DING Xiang-dong，ZHANG Qin，WANG Zhi-gang，WANG Chang-cun，QU Yun-long . Estimation of Genetic Parameters by Connecting Groups for Reproductive Traits of Yorkshire，Landrace and Duroc Pigs in China [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 429-438.
[8]	HONG Qian-long，ZHANG Ying，CAO Chun-wei，WANG Xian-long，ZHAO Jian-guo. Analysis of Sequence，Tissue Expression and Vector Construction of OCA2 Gene in Bama Miniature Pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 439-448.
[9]	XU Pan，ZHANG Zhen，CUI Lei-lei，YANG Bin，DUAN Yan-yu. A Systems Genetics Study of Hematological Traits in a White Duroc × Erhualian Pigs F₂ Resource Population [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 232-240.
[10]	YANG Hui，FANG Shao-ming，HUANG Xiao-chang，CHEN Cong-ying，ZHANG Zhi-yan. Genome-Wide Association Study on Maternal Infanticide Behavior in Pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 241-248.
[11]	LI Man-man，WEN Bing-yan，ZHU He-shui，ZHONG Kai，YANG Guo-yu，WANG Yue-ying. Cloning of Porcine lipasin Gene and Its Effect on LPL Gene Transcription in Liver Cell [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 411-416.
[12]	WANG Chen，QIN Ke，XUE Ming，MO De-lin，CHEN Yao-sheng，LIU Xiao-hong. Application of Genomic Selection in Swine Breeding [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 1-9.
[13]	WANG Shou-dong，FANG Guo-feng，ZENG Yong-qing，CHEN Wei，LI Chuan-hao，WANG Yan-dong. Preparation and Study of CuZnSOD Gene Transgenic Pigs in F₁ Generation [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 16-24.
[14]	ZHANG Dong-jie，LIU Di，ZHANG Xu，WANG Liang，YANG Guo-wei. Study of Pig MSTN Gene Point Mutation Based on the CRISPR-Cas9 System [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 207-212.
[15]	CHEN Chen，HU Xiong-gui，ZHU Ji，REN Hui-bo，DENG Yuan，PENG Ying-lin. Progress on the Research of miRNAs Associated with Fat Development in Pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(12): 2117-2126.

Molecular Cloning and Expression Patterns Analysis of the Porcine STARD3 N-terminal like Protein(STARD3NL) Gene

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments