ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (9): 1830-1839.doi: 10.11843/j.issn.0366-6964.2016.09.011

Previous Articles     Next Articles

Comparative Transcriptome Analysis between Yak and Cattle Estrus Ovary

LAN Dao-liang 1,2,XIONG Xian-rong 2,CHAI Zhi-xin 1,AI Yi 1,HUANG Cai 2,LI Jian 1,2*   

  1. (1.Institute of Qinghai-Tibetan Plateau,Southwest University for Nationalities,Chengdu 610041,China;2.College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041, China)
  • Received:2016-03-31 Online:2016-09-23 Published:2016-09-23

Abstract:

For elaborate the general physiological molecular features of the estrus ovary of yaks and particularity of yak reproduction.RNA-seq technology was applied to analyze transcriptome data comparatively between the yak and plain cattle estrus ovaries in this study.Compared with cattle,genome-wide divergent expression patterns during the ovary transcriptome data revealed that 1 307 genes were significantly and differentially expressed,in which 661 genes were upregulated and 646 genes were downregulated.Functional analysis showed that the differentially expressed genes were involved in various GO categories and KEGG pathways.GO annotations indicated that the genes were closely related to cell adhesion,hormonal biological processes,whereas the calcium ion binding,cation transmembrane transport molecular events were significantly active.KEGG pathway analysis showed that the complement and coagulation cascade pathway was the most enriched,followed by the cytochrome P450 related pathways.Moreover,several novel pathways,such as circadian rhythm,were significantly enriched despite having no evident associations with the reproductive function.This study is the first to compare the ovary transcriptome data between yak and cattle and to identify and analyze the related differential genes.Our findings provide a theory support for further investigation of the molecular mechanism of yak ovary and offer new insights to understand comprehensively the specificity of yak reproduction.

CLC Number: