IBDV感染雏鸡外周血液淋巴细胞chMDA5信号通路因子表达的动态变化

doi:10.11843/j.issn.0366-6964.2015.05.020

Abstract

Abstract:

The present study investigated the expression of chick melanoma differentiation associated gene 5（chMDA5） and its signaling pathway factors in chickens infected with infectious bursal disease virus（IBDV）.Fourty 14-day-old specific pathogen free（SPF） chickens were randomly allocated into 2 groups which were infected and control groups.The infected group of chickens was inoculated with IBDV by intraocularly and intranasally.Meanwhile，the control group chickens were administered with PBS by intraocularly and intranasally.Peripheral blood lymphocytes were isolated at the 1st，4th，7th and 21st day after infection.Real-time fluorescence quantitative PCR method was used to detect the mRNA expression levels of chMDA5 signal pathway factors and the IBDV loads in peripheral blood lymphocytes of chickens.The results showed that the viral loads of IBDV-infected chickens in peripheral blood lymphocytes on the 4th day were significantly higher than that in the 1st day，and then gradually declined.The expression of chMDA5 and its downstream targets genes were significantly increased（P<0.01or P<0.05） at the 4th day after infection，then reduced at 7-21 day than that in the control group.The results indicated that IBDV could activate the chMDA5 signaling pathways in chickens.In addition，the replication of IBDV had closely relationship with the expression of chMDA5 signaling pathways factors.

CLC Number:

S852.659.4

WANG Cheng-cheng，GE Ming，LIU Chao-nan，LUAN Ya-nan，LI Guang-xing，ZHANG Rui-li. Dynamic Changing in the Expression of chMDA5 Signal Pathway Factors in Peripheral Blood Lymphocytes of Chicken Infected with IBDV[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(5): 830-835.

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Dynamic Changing in the Expression of chMDA5 Signal Pathway Factors in Peripheral Blood Lymphocytes of Chicken Infected with IBDV

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