β-1,3/1,6-葡聚糖对肠炎沙门氏菌感染Caco-2细胞抗氧化功能的影响

doi:10.11843/j.issn.0366-6964.2014.10.011

Abstract

Abstract:

In this study，the experiment was conducted to investigate the effects of beta-1,3/1,6-glucan on anti-oxidative functions of Caco-2 cells infected by Salmonella enteritidis.In the experiment，Caco-2 cell line was used as an in vitro model.Caco-2 cells were treated by MEM(group A)，beta-1,3/1,6-glucan(group B)，Salmonella enteritidis(group C)，respectively；Caco-2 cells in group D were pre-incubated with β-1,3/1,6-glucan and then infected with SE.The results showed that the T-AOC concentration significantly increased in group C at 3 and 12 h in cell culture supernatant(P＜0.05).Compared with the control group，the MDA concentration decreased after Caco-2 cells treated 3 h，while enhanced at 12 h(P＜0.01) in cell culture supernatant in group C.Pre-treated with beta-1,3/1,6-glucan increased significantly the total antioxidative capacity of T-AOC in cultured supernatant of Caco-2 cells at 12 h（P＜0.01）and had the tendency to decrease the MDA concentration and increase the SOD activity in cultured supernatant.And the GSH concentration decreased in group D in cell lysate at 12 h.These results suggested that beta-1,3/1,6-glucan can enhance the anti-oxidative function of Caco-2 cells challenged with SE by increasing the T-AOC activity and cutting down the MDA concentration.

CLC Number:

S816.3

LIU Hong-bin，WU Wen-yu，LI Xiao-min，GUO Yu-ming，WANG Zhong. Effect of Beta-1,3/1,6-glucan on Anti-oxidative Functions of Caco-2 Cells Infected by Salmonella enteritidis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(10): 1656-1662.

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Effect of Beta-1,3/1,6-glucan on Anti-oxidative Functions of Caco-2 Cells Infected by Salmonella enteritidis

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