共表达3种纤维素降解酶的转基因小鼠模型的建立与分析

doi:10.11843/j.issn.0366-6964.2016.06.011

Abstract

Abstract:

By expressing fibrolytic enzymes targeting cellulose and xylan through transgenic technology，new breeds of monogastric animals with enhanced feed conversion rates could be developed.Here we described the co-expression of cellulase genes EGXA and BGL4，and the xylanase gene XYNB，under the control of EF1α promoter and linked via 2A peptides，in both HEK293A cells and transgenic mice.Recombinant enzyme expression was detected in gastrointestinal tract as determined by both Western blot and immunohistochemistry，and were functionally active in cellulolytic enzyme assays.Notably，all 3 fibrolytic enzymes were highly expressed in the intestine tissue of transgenic mice.In summary，we have demonstrated that the EF1α promoter in conjunction with 2A peptide sequences could successfully mediate the co-expression of EGXA，BGL4 and XYNB genes in transgenic mice.The study presents a feasible method for generating genetically modified animals with enhanced feed conversion ability.

CLC Number:

HE Zu-yong，LIU Zhi-guo，SUN Yan-xia，CONG Pei-qing，CHEN Yao-sheng. Generation of a Transgenic Mouse Model Co-expressing Three Fibrolytic Enzyme Genes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(6): 1162-1169.

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Generation of a Transgenic Mouse Model Co-expressing Three Fibrolytic Enzyme Genes

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