浏阳黑山羊胃肠道不同部位重要功能微生物的数量分布特征研究

doi:10.11843/j.issn.0366-6964.2013.10.012

Abstract

Abstract:

The aim of this study was to explore the quantity and distribution characteristics of functional microorganisms in gastrointestinal tract of Liuyang Black goats, and provide foundation for further study on nutritional metabolism of these microbes. Microbes with known definite functions, such as bacteria and methogen, four cellulolytic bacteria, probiotics (Lactobacillus and Bifidobacterium), Prevotella ruminicola were selected in this study. Four Liuyang Black goats (1.5-year-old, (20.0±1.2)kg weight) were slaughtered at the same time and the digesta in the duodenum, jejunum, ileum, cecum, colon, rectum and rumen were collected for the isolation of the DNA, followed by the construction of the recombined standard plasmids. qRT-PCR was used for the absolute quantification of the microbes. The standard plasmids and curves of the microbes and methogen were constructed successfully and could be applied to the research. There were significant differences (P<0.01) in microbes between the 7 sections of the gastrointestinal tract of Liuyang Black goats. And the numbers of the microbes in the same section differed from each other significantly. Apart from the Butyrivibrio fibrisolvens, the largest number of the microbes was found in the rumen. In the intestinal tract, the number of microbes in the duodenum and jejunum was the least, while the number of microbes in the cecum, colon and rectum was the largest. The quantity and distribution characteristics of functional microorganisms in different gastrointestinal tracts of Liuyang Black goats significantly differ from each other (P<0.01). Overall, the number in the rumen was the biggest; the number in the cecum, colon and rectum was middle, followed by the ileum, while the least number was found in the duodenum and jejunum. This difference in the quantity and distribution characteristics of functional microorganisms in gastrointestinal tract has paved a way for the further study on different nutritional metabolism of these microbes.

CLC Number:

S827
S811.2

JIAO Jin-zhen, WANG Peng-peng, TANG Shao-xun, ZHOU Chuan-she, TAN Zhi-liang. Quantity and Distribution Characteristics of Functional Microorganisms in Gastrointestinal Tract of Liuyang Black Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(10): 1590-1599.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2013.10.012

https://www.xmsyxb.com/EN/Y2013/V44/I10/1590

References

［1］JENKINS T C, WALLACE J R, MOATE P J, et al. Recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem ［J］. J Anim Sci, 2008, 86: 397-412.

［2］HOOPER L, GORDON J. Commensal host-bacterial relationships in the gut ［J］.Science, 2001,292:1115-1118.

［3］冯仰廉. 反刍动物营养学［M］. 北京: 科学出版社, 2004:131-132.

［4］CUMMINGS J H, MACFARLANE G T. Role of intestinal bacteria in nutrient metabolism ［J］. Clin Nutr, 1997: 16:3-11.

［5］邢廷铣, 陈凯凡. 肉皮兼用型特色羊—浏阳黑山羊［J］. 农村百事通, 2005,(3): 43.

［6］刘宗棠. 浏阳黑山羊被列为国家保种项目［J］.湖南农业, 1999, (10): 37.

［7］陈幼春. 牛的品种、高档牛肉种类和生产因素［J］. 黄牛杂志, 1995, (01): 37-40.

［8］茆达干, 石国庆, 张红琳等. 湖羊早期妊娠的子宫内环境研究［J］. 畜牧兽医学报,2010,41(9): 1203-1207.

［9］HOOK S E, NORTHWOOD K S, WRIGHT D G, et al. Long-term monensin supplementation does not significantly affect the quantity or diversity of methanogens in the rumen of the lactating dairy cow ［J］. Appl Environ Microbiol, 2009, 75(2): 374-380.

［10］DENMAN S E, MCSWEENEY C S. Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen ［J］. FEMS Microbiol Ecol, 2006, 58:572-582.

［11］STEVENSON D M, WEIMER P J. Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR ［J］. Appl Microbiol Biotechnol, 2007, 75(1): 165-174.

［12］KOIKE S, KOBAYASHI Y. Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefacies ［J］. FEMS Microbiol Lett, 2001, 204: 361-366.

［13］王加启．反刍动物营养学研究方法［M］. 北京:中国出版集团现代教育出版社,2011:32-33.

［14］ZHOU M E, SANABRIA E H, LE L G. Assessment of the microbial ecology of ruminal methanogens in cattle with different feed efficiencies ［J］. Appl Environ Microbiol, 2009, 75(20): 6524-6533.

［15］MACKIE R I, WHITE B A. Recent advances in rumen microbial ecology and metabolism: potential impact on nutrient output ［J］. J Dairy Sci, 1990, 73(10): 2971-2995.

［16］RUSSELL J B, RYCHLIKJ L. Factors that alter rumen microbial ecology ［J］.Science, 2001, 292(11): 1119-1122.

［17］孔祥浩, 贾志海, 郭金双. 反刍动物后肠道对碳水化合物的消化吸收研究进展［J］.中国畜牧杂志, 2005,41(2):36-38.

［18］赵玉华. 瘤胃微生物Real Time PCR 定量方法的建立及其应用［D］. 北京: 中国农业科学院, 2005.

［19］TAN Z L, LU D X, HU M, et al. Effect of dietary structural to nonstructural carbohydrate ratio on rumen degradability and digestibility of fiber fractions of wheat straw in sheep ［J］. Asian-Austra J Anim Sci, 2002, 15(11): 1591-1598.

［20］VAN DER WERF M J, VENIMA K. Bifidobacteria: genetic modification and the study of their role in the colon［J］. J Agric Food Chem, 2001, 49(1): 378-383.

［21］黄沧海, 谯仕彦, 李德发. 益生乳酸杆菌抑制大肠杆菌的研究［J］. 中国畜牧杂志, 2003, 39(6): 27-28.

［22］COLLINS M D, GIBSON G R. Probiotics, prebiotics and synbiotics: approaches for modulating the microbial ecology of the gut ［J］. Am J Clin Nut, 1999, 69: 1052S-1057S.

［23］盛清凯, 姚惠源. 低聚糖对肠道菌群的调节机理［J］.动物科学与动物医学,2002, 19(2): 35-38.

［24］WANAPAT M, CHERDTHONG A. 2009. Use of real-time PCR technique in studying rumen cellulolytic bacteria population as affected by level of roughage in swamp buffalo ［J］.Curr Microbiol, 2009, 58: 294-299.

[1]	YAN Xiaochun, XI Haijiao, LI Jinquan, WANG Zhiying, SU Rui. Study on Estimates of Genomic Breeding Value of Fleece Traits in Inner Mongolia Cashmere Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 120-128.
[2]	ZHANG Chenjian, LI Yinxia, DING Qiang, LIU Weijia, WANG Huili, HE Nan, WU Jiashun, CAO Shaoxian. Efficient Preparation of CRISPR/Cas9-mediated Goat SOCS2 Gene Edited Embryos [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 129-141.
[3]	MENG Qiuchi, LU Guangyu, CHEN Dingshuang, LIN Yaqiu, WANG Ruilong, ZHONG Chaosong, WANG Yong, LIU Wei, WANG Youli, LI Yanyan, LI Zhixiong. Goats GPR35 Gene Expression Characteristics Analysis and the Effect on Subcutaneous Fat Cell Differentiation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4993-5007.
[4]	HE Zhicheng, QIN Xiaochen, Lü Yongqiang, LI Xiujin, BIAN Huilong, LUO Jun, LI Cong. Multiple Stepwise Regression Analysis and Growth Curve Fitting of Body Measurement Traits in Saanen Dairy Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5301-5311.
[5]	ZHANG Renbao, ZHOU Donghui, ZHOU Lisheng, GAO Xiaoxiao, LIU Nan, HE Jianning. Analysis of Genetic Structure of Conservation Population in Jining Gray Goats Based on 70 K SNP Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2836-2847.
[6]	SHAO Yuexin, ZHANG Xinyu, GE Liyan, SHI Huaiping. Cloning and RNA Interference Analysis of ATF4 Gene in Xinong Saanen Dairy Goat [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2353-2364.
[7]	LIU Jie, XU Xiangping, DENG Ming, ZOU Xian, JIANG Shengwei, LIU Dewu, LIU Guangbin, SUN Baoli, GUO Yongqing, LI Yaokun. Effect of miR-144-5p Targeting WNT5a on the Proliferation and Apoptosis of Goat Ovarian Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2421-2435.
[8]	XU Tiantian, ZHANG Tongtong, WANG Meng, WANG Xin. Transcription Factor Foxq1 Affects the Proliferation of Hair Follicle Stem Cells in Cashmere Goats via WNT/β-catenin Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2653-2661.
[9]	QUBI Wuqie, LI Yanyan, LI Xin, WANG Yong, WANG Youli, LIU Wei, ZHU Jiangjiang, LIN Yaqiu. APOA4 Gene Inhibits Intramuscular Adipocyte Differentiation in Goat [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1927-1938.
[10]	MA Keyan, HAN Jintao, BAI Yaqin, LI Taotao, MA Youji. Genetic Diversity Analysis of Yongdeng Qishan Sheep Based on Specific-Locus Amplified Fragment Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1939-1950.
[11]	WANG Li, GUO Yaru, ZHANG Junmei, LEI Mingkai, WANG Zhenguo, ZHANG Chunxiang, REN Youshe. Effect of 1,25(OH)₂D₃ and VDR Knockout on the Expression of β-defensins Family in the Caprine Epididymal Caput Epithelial Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1990-2000.
[12]	ZHU Junru, WEI Wei, PEI Dangshuai, ZHANG Fenque, DUAN Yu, GUO Yongfeng, JIANG Yue, XIA Shuli, HAN Jing, HOU Jinxing, AN Xiaopeng. Effects of PDCD4 on the Apoptosis of Dairy Goat Mammary Epithelial Cells and the Synthesis of β-casein and TG [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1429-1440.
[13]	CHEN Cancan, JIANG Jing, SUN Xiaoyan, REN Hangxing, LI Jie. Expression of AGRP Gene in Goat Tissue and Its Action Mechanism on Melanin Production [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1441-1451.
[14]	DAN Yixin, YANG Lu, XIANG Hua, ZHANG Huanrong, REN Yupeng, YANG Falong, HE Honghong, ZHU Jiangjiang. Effects of BIRC5 on the Cycle and Apoptosis of Goat Testis Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1511-1524.
[15]	YE Junning, DENG Ming, XUE Huiwen, LIU Guangbin, ZOU Xian, SUN Baoli, GUO Yongqing, LIU Dewu, LI Yaokun. Identification and Analysis of mRNA and lncRNA Affecting Goat Fetal Muscle Development [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 989-1002.

Quantity and Distribution Characteristics of Functional Microorganisms in Gastrointestinal Tract of Liuyang Black Goats

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments