绒山羊肌内前体脂肪细胞的基因表达分析

doi:10.11843/j.issn.0366-6964.2013.10.004

Abstract

Abstract:

The intramuscular preadipocytes culture model in vitro of goat was established in this study, in order to research marker gene expression in intramuscular adipose tissue development and fat deposition in goat. The longissimus muscle of 1-day-old lamb was collected in this experiment, and digested by type II collagenase for 1.5 h, then filtered by 80 mesh, 400 mesh in sequence, after centrifugation, proliferative intramuscular preadipocytes were obtained using differential adhesion method. The morphological changes were observed, and fat content in cells was detected by oil red O staining. The genes expression of LPL, PPAR, FTO and LIPIN was detected using real-time quantitative PCR. The results showed that: the primary cultured cells were homogeneous, and the shortening-shaped or prismatic cells were showed in adherent growth, with the morphological characteristics of the intramuscular preadipocytes. The mRNA of FTO gene had a higher expression level in early stage, and the peak occurred at the 5-7 d in differentiation, and thereafter maintained a high level, then expression amount significantly decreased till 11 d (P<0.05). LIPIN mRNA fluctuated after the addition of inducer. PPAR mRNA maintained a relatively stable level in whole differentiation and proliferation process. LPL mRNA was expressed highly in the early stages of differentiation, and the expression decreased gradually with the increase of the degree of differentiation. The primary culture method of intramuscular preadipocytes in goat was established successfully in this research, and proliferation and differentiation process were reproduced in vitro. The amount of gene expression by quantitative analysis was: FTO> LPL> PPAR> LIPIN. This research laided the basis for further study of the fat deposition mechanism of intramuscular adipose tissue and improving the quality of goat.

CLC Number:

S827
S813.3

DU Chen, FU Shao-yin, HAN Zhi-ling, MENG Li-yun, AILUN Gao-wa, GAO LI-xia, CHENG Li-xin, ZHANG Wen-guang,LI Jin-quan. Gene Expression Analysis on Intramuscular Preadipocytes in the Cashmere Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(10): 1532-1538.

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Gene Expression Analysis on Intramuscular Preadipocytes in the Cashmere Goats

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