老龄牦牛睾丸细胞外基质相关蛋白的分布特征

doi:10.11843/j.issn.0366-6964.2015.09.023

Abstract

Abstract:

This study was conducted to characterize the structure and distribution of extracellular matrix(ECM) proteins of testis in aging yak testis.The testis from 9 aging and 10 young adult yak were prepared for distribution investigation of laminin(LN)，type IV collagen(ColⅣ) and heparan sulfate proteoglycans(HSPG) by immunohistochemistry and histochemistry methods.The observations made with the light microscope showed obvious morphological changes of aging，there were partly progressive regressed in the seminiferous epithelium and the volume of interstitial tissues has further increased and abundant with collagen and reticular fiber，and also there were AB-PAS positive stain in aging yak seminiferous basement and blood capillary.Statistical data showed that the number of Sertoli cells and Leydig cells in aging yak testis were reduced obviously，but the cross-sectional area of the seminiferous tubules and average interstitial tissue areas were more larger than which in the young yak testis.Immunostaining analysis appeared that the LN was present similarity in Sertoli cells and peritubular myoid cells in the aging and young adult yak.The relative expression of LN in spermatogenic cells was decreased and almost no detected in aging yak Leydig cells(P<0.01).By contrast，the distribution of Col Ⅳ without statistical differences in yak testis at different ages.The relative expression of HSPG in the aging yak testis tissue was more lower than which in young adult yak，and the strong immunoreactivity for HSPG was seen in the Leydig cells(P<0.01).In addition，the average absorbance of LN，Col Ⅳand HSPG expression was no obvious difference between different ages.All the elements of the intralobular interstitial space may undergo degenerative changes in aging yak which live in plateau environment，the secretion of Col Ⅳ increased company with the synthesis of collagen fiber reinforced，and the distribution of ECM proteins LN and HSPG were significantly decreased may closely related with the alterations of Leydig cells′ synthesis and secretion ability.

CLC Number:

S852.162

YUAN Li-gang，ZHU Jun-feng，GU Lai-feng，CHEN Guo-juan. Distribution of Extracellular Matrix Components in Aging Yak Testis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1656-1662.

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Distribution of Extracellular Matrix Components in Aging Yak Testis

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