内蒙古地区猪腹泻相关病毒的检测与分析

doi:10.11843/j.issn.0366-6964.2015.09.018

Abstract

Abstract:

The research aimed to understand the main pathogens of pigs diarrhea virus found in Inner Mongolia in order to provide theoretical basis for pig diarrhea control and prevention.Through the Multiple RT-PCR method，the porcine epidemic diarrhea virus (PEDV)，transmissible gastroenteritis virus (TGEV)，and porcine rotavirus (PoRV) from 394 tissue samples collected from the pig farms in 8 cities of Inner Mongolia were tested.M gene of the PEDV positive materials were cloned and analyzed along with the sequence alignment and phylogenetic trees of 48 M gene sequences from other domestic and international.The tested results indicated that the positive rates of PEDV and PoRV were 63.96% and 2.03%，respectively，yet the TGEV tested negative，The Mixed infection rate of PEDV and PoRV was 2.03%.Phylogenetic analysis indicated that CH/NMG/ XLGL strain stayed close relative with classic strains such as CV777，located at G2.The rest of the strains stayed close relatives with the domestic many provinces before 2012，and Thailand，South Korea，Russia，Vietnam and other neighboring countries were same branch，G1-1.United States，in particular，the nucleotide sequence homology was 99.5%-100%.From 2013 until now，the main pathogeny for pig diarrhea in Inner Mongolia was PEDV，followed by PoRV.The infection degree was different in varying cities，and pigs of all ages can be infected，especially the suckle pigs and the sow.Most of the prevalent strains exhibited higher affinity with the strains isolated from the most provinces and cities domestic since 2012，but the stains were far apart from Inner Mongolia strains isolated before 2007，vaccine strains and classic strains，and some sites appeared base mutations.

CLC Number:

S858.285.3

LIU Yan-cheng，DU Ya-nan，WU Wei-jie，WANG Xue-fei，LU Ting，LIU Dan-dan. Detection and Analysis of Porcine Diarrhea Associated Virus in Inner Mongolia[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1620-1628.

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Detection and Analysis of Porcine Diarrhea Associated Virus in Inner Mongolia

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