Abstract:

 Eukaryotic expression vectors of mouse Fyn and its different mutants were constructed. Structural and functional changes of their protein products were predicted. Fyn cDNA from mouse cerebral cortex was cloned and subcloned into pMD18-T. Validated pMD18-T-Fyn^wt was used as template to construct clone plasmids of different mutants using different primers. The validated Fyn^wt and its different mutants were cloned into pCAG-MCS to get the eukaryotic expression vector pCAG-MCS-Fyn^**. Characteristics of their bioinformatics were analyzed. The complete sequence showed that the cDNA sequence of Fyn^wt is identical with that in GenBank. The fragments of Fyn mutants have fully acquired as expectation. The relative expression level of multiple Fyn in cell rised obviously （P<0.01）. Bioinformatics analysis showed that the secondary structure of protein products of Fyn mutants changed remarkably by comparison with that of Fyn^wt, which may influence the bioactivity of Fyn.