红景天多糖对猪卵母细胞体外成熟及其发育潜力的影响

Abstract

Abstract:

The study was designed to investigate the effect of Rhodiola sachalinensis polysaccaride (RSP) on developmental competence of porcine oocytes. Immature oocytes were matured in vitro and supplemented with different concentrations of RSP, the extrusion of first polar body was treated as a symbol of nuclear maturation, then the nuclear maturation rates, oocyte intracellular glutathione (GSH) content and reactive oxygen species (ROS) level, embryonic development after parthenogenetic activation (PA) and in vitro fertilization (IVF) were conducted. The result showed that the nuclear maturation rate in each RSP treatment were higher than that of control group (83.50%±1.00%, P>0.05); The GV stage oocytes treated with 6.0 and 60.0 mg·L^-1 RSP in vitro maturation medium could significantly increase the GSH content (P<0.05), and decrease the ROS level (P>0.05); After PA, the cleavage rate of 60.0 mg·L^-1 RSP (88.44%±5.06%) and the blastocyst rate of 6.0 mg·L^-1 RSP (40.94%±8.23%) were the highest,and were significantly higher than those of control group (72.16%±7.38%, 24.06%±5.16%,P<0.05); IVF cleavage rate in each RSP treatment was significantly increased (P<0.05), IVF blastocyst rate (19.20%±3.13%) derived from oocytes treated with 60.0 mg·L^-1 RSP was significantly higher than that of control group (9.60%±0.65%, P<0.05). The results showed that adding RSP in vitro maturation medium could increase nuclear maturation rate, promote cytoplasm maturation, and then increase the developmental competence of porcine oocytes after PA and IVF.

CLC Number:

S828.3

XU Li, DAI Jian-jun, ZHANG Ting-yu, WU Cai-feng, ZHANG Shu-shan, ZHANG De-fu, GU Xiao-long, LI Xiang, HAN Xiao, LI Fang-fang. Effect of Rhodiola Sachanensis Polysaccharide (RSP) on Developmental Competence of Porcine Oocytes Following Maturation and Culture in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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Effect of Rhodiola Sachanensis Polysaccharide (RSP) on Developmental Competence of Porcine Oocytes Following Maturation and Culture in vitro

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