ACTA VETERINARIA ET ZOOTECHNICA SINICA

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Effect and Mechanism of Lipopolysaccharide on Distribution of Plasma Free Amino Acid in Dairy Goats Fed High Concentrate Feed

ZHANG Shu-kun, JIANG Xue-yuan, XIE Zheng-lu, NI Ying-dong, ZHUANG Su,ZHANG Yuan-shu*, SHEN Xiang-zhen   

  1. (Key Laboratory of Animal Physiology and Biochemistry of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China)
  • Received:2012-07-30 Online:2013-03-23 Published:2013-03-23

Abstract:

The aim of this experiment was to explore the effect and mechanism of LPS on distribution of free amino acid of plasma in dairy goats fed high concentrate feed. Six dairy goats during the dry period ,which were installed permanent rumen fistulas, were randomly divided into two groups including low concentrate group(concentrate-to-forage ratio 4∶6)and high concentrate group(concentrate-to-forage ratio 6∶4).Using a crossover experimental design divided into two phases, each phase was fourteen days. Rumen fluid pH , the content of LPS and free amino acids were determined in the first three weeks of the experiment. The results showed that those goats fed with high concentrate began to appear subacute ruminal acidosis(SARA) from the third week, and their LPS contents in peripheral blood significantly increased (P<0.05). The content changes of each amino acid in the plasma also were found. Compared with low concentrate group, both Glu and Ala contents were significantly lower (P <0.01), Gln, Arg, Thr, Lys, total non-essential amino acids, total free amino acids and total glucogenic amino acids significantly decreased (P<0.05). These results indicated that amino acids in plasm of goats appeared to distribution as the increased the proportion of concentrated feed in the diet. Its mechanism may be related to the decrease of pH in rumen and the increase of LPS in blood.

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