Abstract:

Full length coding sequence of porcine Prox1 gene was cloned from liver cDNA. Then the chromosomal and subcellular localization of sProx1 were investigated. In this study, Blast searches was employed to obtain homologous porcine ESTs in the porcine EST databases with the Prox1 gene sequence of human and mouse, primers were designed and the mRNA sequence of Prox1 gene was amplified by RT-PCR. Blasted the intron2 sequence of Prox1 between human, mice and other kinds of species, a pair of primers was designed for the partly cloning of porcine Prox1 intron2. Chromosome localization of Prox1 was detected by IMpRH (INRA-Minnesota porcine radiation hybrid panel). pEGFP-N1-Prox1 was constructed and PK15 cells were transfected with the plasmid. The subcellular localization of Prox1 protein observed by inverted fluorescence microscope after 12 h. The complete Prox1 cDNA sequence and the intron2 partial sequence was cloned（GenBank Accession NO.EF486324，EF571585）. sProx1 gene was physically mapped on porcine chromosome 9q26 and closely linked to marker SW1651, and recombinant plasmid pEGFP-N1-Prox1 was constructed and EGFP-Prox1 was expressed in the nucleus of PK15 cells.