Abstract: The objective of this study was to understand the dynamic distribution pattern of G. anatis in chicken and the effect of chicken infectious bronchitis virus (IBV) on the distribution pattern. In the present study, SPF layer chickens were inoculated with G. anatis or/and chicken infectious bronchitis virus (IBV). Quantitative PCR (qPCR) with SYBR Green I was used to detect the DNA of G. anatis in different organs at different time point from chicken after artificial infection. In G. anatis group, G. anatis DNA was firstly detected in the trachea, heart, spleen, ovary and kidney of chickens 12 hours post inoculation (PI), in the liver, duodenum and oviduct 24 hours PI, in the lung 48 hours PI, and in the palate cleft 72 hours PI, respectively. In-mixed infection group, G. anatis DNA was detected 12 hours PI in the ovaries, and 24 hours PI in all the organs. The highest content of G. anatis DNA was noticed in ovary and the highest detection rate was found in trachea of birds. Mixed-infection group had significantly higher qPCR detection rate of all organs than G. anatis group. G. anatis could cause systemic infection of the birds, and coinoculation of G. anatis and IBV increased the systemic infection. IBV promoted the spread of G. anatis, moreover, IBV benefited the multiplication and pathogenicity of G. anatis in the duodenum and thus resulted in clinical diarrhea. It was further confirmed that the pathogenicity of G. anatis mainly focus on the respiratory and reproductive systems. Trachea and ovaries were the target organs of G. anatis.