Abstract: The objective of this study was to investigate the positive rate for antigen P27 of Avian leukosis virus in eggs from different breeder groups and its correlation with the incidence of avian leukemia both in the breeder chickens and its next generation. It is hoped that this study will be helpful for the breeders to estimate the risk of avian leukosis virus infection. Eggs or sera samples from 7 domestic breeder groups from Tianjin, Shandong, Hubei, Hebei province were collected and detected for antigen P27 of ALV and antibodies to ALV-A/B and ALV-J respectively. Some samples with tumors were isolated for exogenous ALV. The tumor report and complaint for the next generation of each breeder were tracted and analyzed. The positive rate of antigen P27 in eggs of different breeders is quite different and the positive rate range from 0 to 12%, positive rate for antigen P27 in eggs closely related to the incidence of ALV infection both for the breeders and its next generation. That is, the higher it is, the more serious for ALV infection and the more complaints from customers. The positive rate for antigen P27 in eggs of breeders conform well to virus isolation and tumor incidence and can be used as an important index to estimate the risk of ALV infection.