Abstract: This research tried to detect the relative expression level of mRNA and protein of ALB gene in livers, pancreases, lungs, kidneys, cerebra, cerebella, leg muscles and thymuses infected by duckling hepatitis virus (DHV1). The expression level of ALB gene and the content of ALB protein of control group, susceptible group and resistant group were detected by realtime quantitative RTPCR and ELISA techniques. Generally speaking, except in leg muscles, the expression level of ALB genes mRNA in susceptible group was highly significantly less than those in control group and resistant group (P<0.01), and the level in resistant group was significantly or highly significantly less than that in control group (P<0.05 or P<0.01). And the content of ALB protein has the same differences as ALB gene's mRNA in livers, cerebella and thymuses among those three groups, but there were no significant differences in the other tissues(P>0.05). It was showed that the results of RTPCR and ELISA techniques had the same results in the indicative tissues directly connecting with duck hepatitis such as livers, thymuses, cerebella, etc. This research gets the same results as those using suppression subtractive hybridization (SSH) and makes a further step to reveal that ALB gene is the resistant gene of duck hepatitis, whose change of the expression level can be used as a marker to distinguish susceptible and diseaseresistant ducks.