ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (5): 767-772.doi:

• 预防兽医 • Previous Articles     Next Articles

Development and Application of TaqMan Fluorescent Realtime Quantitative PCR for the Detection of Egg Drop Syndrome Virus

MA Zhenyuan1,2, LI Gang2*, LI Wenchao2, GUO Yufei1,3   

  1. 1.College of Animal Science and Veterinary Medicine, Sichuan Agricultural University， Ya’an 625014, China; 2.Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100093, China; 3. College of Animal Science and Veterinary Medicine, Jinling Institute of Technology, Nanjing 210038, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-05-28 Published:2012-05-28
  • Contact: LI Gang

PDF (PC)

530

Abstract: To establish the fluorescent realtime quantitative PCR (FQRTPCR) assay for rapid detection of egg drop syndrome virus, a pair of primers and a TaqMan probe were designed according to the sequences from highly conserved regions of the hexon protein gene of egg drop syndrome virus (EDSV). A positive recombinant plasmid was employed as standard template for the construction of standard curve. The detection limit of the assay was 10 copies DNA·μL-1 and the FQRTPCR was reproducible, as shown by satisfying its wide dynamic range from 101 to 108 copies DNA·μL-1. This assay is specific and has no crossreaction with DNA of other avian virus. The quantity of EDSV in eggs after artificial infection with NE4 strain of EDSV were detected by using the developed FQRTPCR method. Compared with common PCR assay, the FQRTPCR method is more sensitive and more suitable for detection of egg drop syndrome virus in vivo and in vitro.