ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2010, Vol. 41 ›› Issue (4): 449-453.doi:

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Evaluation of Three Serological Tests for Brucellosis in Cattle Using Bayesian Analysis"

LIU Xiaomin, YANG Hua, GAO Mingchun, LI Yanwei, WANG Junwei*
  

  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China
  • Received:2009-10-22 Revised:1900-01-01 Online:2010-04-25 Published:2010-04-25
  • Contact: WANG Junwei

Abstract: The objective of our study was to evaluate the diagnostic performance of three serological tests for brucellosis. Serological test results were used to compare the performance of Rose Bengal Test (RBT), indirect ELISA (IELISA) and Fluorescence Polarisation Assay (FPA) in diagnosing brucellosis in cattle. Their performance was evaluated using Bayesian analysis which allows the sensitivity (Se) and specificity (Sp) to be estimated in the absence of a gold standard. The sensitivity of RBT, FPA and IELISA were 91.81%, 95.92% and 95.98%, respectively; and specificity of RBT, FPA and IELISA were 98.80%, 86.54% and 98.65%, respectively. The results demonstrated that the IELISA performing well in diagnosing brucellosis in cattle.