ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (7): 885-890.doi:

• 遗传繁育 • Previous Articles     Next Articles

Restriction Fragment Length Polymorphism in MC4R Gene and ItsAssociation with Carcass and Feather-down Traits in Geese

CHEN Hong-quan*,HUANG Hua-yun, CHEN Hua, ZHANG Tong-yan   

  1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-07-23 Published:2008-07-23

Abstract: The carcass traits, meat quality traits and feather-down traits of 123 geese from F1 population of Wanxi White goose (male parent) and Sichuan White goose (female parent) were mensurated, and 12 common factors were extracted from the traits using factor analysis. The coding region of melanocortin receptor-4 (MC4R) gene was cloned, and the amplified products were digested with four restriction endonucleases Hinf Ⅰ,Ear Ⅰ,PstⅠ and PvuⅡ to detect restriction fragment length polymorphism (RFLP) in MC4R gene and its association with the traits. The results showed that the RFLP emerged only using HinfⅠ with two genotypes HH and Hh, and frequencies of allele H and h were 0.890 2 and 0.109 8 in F1 population respectively The effects of genotype HH were significant on carcass weight, eviscerated weight (with giblet), liver weight, heart weight, stomach percentage, breast fat percentage, chest muscle water loss percentage and down proportion, and all genotypic contribution percentages (genotypic effect by population mean, CP) were between -5% and 5%, but on which the effects of genotype Hh were not significant after MC4R gene mutation. In effects of different genotype on 12 common factors, only the effects of genotype Hh were significant on fat power (CP = 3.314%, P<0.05) and fatty acid quality (CP = 1.218%, P<0.05).