ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (7): 853-857.doi:

• 遗传繁育 • Previous Articles     Next Articles

Analysis of Sequences of BoLA-DRB3 Genes in Chinese Yellow Cattle

WANG Kun1，ZHANG Yuan2，SUN Dong-xiao2   

  1. 1.Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050031, China；2College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-07-23 Published:2008-07-23

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Abstract: In this research, four Chinese Yellow cattle breeds were studied for the molecular genetic diversity in MHC-DRB3*exon2 by DNA sequencing. A total of 259 DRB3*exon2 alleles including 131 newly identified ones were found. The number of alleles represents differences in breeds, indicating genetic variations among different breeds. The estimation of the average rate of synonymous（dS）and non-synonymous（dN）substitution in the exon 2 of BoLA-DRB3 showed there was a higher ratio of dN to dS substitutions in the peptide-binding site (PBS) region. The amino acid components of 17 polymorphic sites were significant difference among breeds, including 8 PBS.