Abstract: The plant seed-specific expression vector p7SBin438/VP1 for VP1 gene of footandmouth disease virus O/China/99 strain was constructed. Mediated with Agrobacterium tumefaciens GV3101 harboring p7SBin438/VP1, VP1 gene was transferred into Arabidopsis thaliana via floral dip. After selecting by Kanamysin, a number of resistant lines were obtained. The presence of VP1 gene in the transformed Arabidopsis thaliana genome was confirmed by PCR. The active detection of expressed target protein in the pods and leaves of transgenic Arabidopsis thaliana was confirmed by sandwich-ELISA and Western blot. Genetic analysis of transgenic progeny was performed by PCR. Experimental results showed that the plant seed-specific expression vector was successfully constructed and the structural protein VP1 was expressed in pods at high-level. Western blot showed that recombinant protein expressed in pods had immunocompetence and could react with FMDV antisera. Progeny test showed that VP1 gene in the transgenic Arabidopsis thaliana was heritable stablely. These results may provide the experimental bases for further research on transformed leguminous plant.