Acta Veterinaria et Zootechnica Sinica

netAssociation of the H-FABP and A-FABP Gene Expression with Intramuscular Fat Content in Chickens

LI Wen-juan;LI Hong-bin;WEN Jie;CHEN Ji-lan;ZHAO Gui-ping;ZHENG Mai-qing

2006, 37(5): 417-423. doi:

Abstract ( 765 )

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Four chicken breeds, including Beijing Fatty, Dwarf, White Leghorn and Arber Acres chickens were used as experimental populations. RT-PCR and real-time fluorescence quantity PCR technique were used to study genetic expression in heart and adipocyte fatty acidbinding protein (H-FABP, A-FABP) genes at 56, 90 and 120 day of age. The associations between genetic expression level and intramuscular fat content and slaughter traits were analyzed by using partial correlated analysis. Statistical analyses showed, for genes expression, the H-FABP mRNA level is significant increasing and A-FABP mRNA level is markedly reducing with age rise. Breeds had remarkably effect on the H-FABP and A-FABP genes expression. Moreover, the sexes had a significant effect on the A-FABP mRNA expression but not H-FABP gene’s. The H-FABP gene expression levels were significantly negative correlation with IMF contents and carcass weight, however, A-FABP mRNA level had a significantly effect on the carcass weight but not IMF content in Beijing Fatty, White Leghorn and Arber Acres chicken . The A-FABP mRNA expression was negatively related significantly with IMF% and H-FABP mRNA level had　marked relation with carcass weight in Dwarf chicken.

Study on the Correlations between RAPD Marker and Phenotypic Traits in the Sichuan Black Goat

LI Li;QIU Dun-lian;HUANG Jian;ZHOU Guang-ming;FU Chang-xiu;LI Qiang;YANG Zhi-rong

2006, 37(5): 424-429. doi:

Abstract ( 1356 )

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16 random primers with sufficient polymorphism and reproducible patterns were selected to amplify the blood DNA from 96 adult Sichuan black goat at age of 1.5 to 2 years old.170 polymorphic RAPD bands were produced with the 16 primers. These bands were recorded in the form of 1 (presence of band) and 0 (absence of band). To a specific marker, the individuals with different polymorphic bands are treated as different samples. The difference of the average for quantitative traits of the two samples were compared with Independent-samples T Test by using SPSS software. Molecular markers having true correlation with traits were selected according to the distribution of the individual phenotypic value of two independent samples. The results showed that 5 molecular markers in the black goat population were related to different phenotypic traits. It is suggested that this marker may have linkage relationship with the quantitative trait loci or the major gene controlling this trait.

Studies on the Polymorphism of SLA-DQA Gene between Diannan Small-ear Pig and Bama Pig

LI Hua;YU Hui;JIANG An-an;LI Xue-wei;TIAN Yun-bo;ZHAO Hai-quan;CHEN Yun

2006, 37(5): 430-435. doi:

Abstract ( 742 )

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The partial intron 1, complete exon 2 and partial intron 2 of SLA-DQA gene was amplified and a uniform fragment of 341 bp was obtained in Diannan small-ear pigs (DN) and Bama miniature pigs (BM). After PCR products were purified and digested by EcoR Ⅰ and Alu Ⅰ, the results showed that the genotype frequency of BB (0.468 8) at EcoR I-RFLP site was higher than that of AB (0.375 0) and AA (0.156 3), respectively. By comparing with the allelic frequency, the predominant allele was B(0.656 3),while the inferior allele was A (0.343 7).To the Alu Ⅰ-RFLP site, the genotype frequency of MM (0.500 0) was higher than that of MN(0.321 4) and NN(0.178 6) in DN, respectively. While the genotype frequency of MN (0.485 3) was higher than that of MM (0.338 2) and NN (0.176 5) in BM, respectively. The allelic frequency of M (0.604 2) was higher than that of N(0.395 8) in two breeds. The results of χ² analysis showed that genotypes of the SLA-DQA gene in two breeds fit Hardy-Weinberg equilibrium. There are nine and seven composite genotype of SLA-DQA gene in BM and DN, respectively. Among them, the most favorable was BBMN, while the inferior was AAMN, the genotype frequency of AAMM were found to be significantly different between two pig breeds（P<0.05）. The results of genetic parameters showed that there were moderate polymorphic in exon 2 of SLA-DQA gene, the polymorphism information content (PIC) at Alu I site was higher than that at EcoR I site, the slightly higher heterozygote existed in BM than that in DN, the Nei’s unbiased genetic distance is 0.000 4 between two breeds.

Study on the Isolation and Culture of Goat Hair Follicle Stem Cells

SHI Ming-yan;YANG Xue-yi;Dou Zhong-ying

2006, 37(5): 436-440. doi:

Abstract ( 1348 )

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Goat hair follicle stem cells were isolated by two different methods of explants culture and enzyme digestion. The resultant cells were cultured in vitro and identified by cell growth curve, immunohistochemical staining and colonyforming efficiency to compare the efficiency of the two methods for the cultivation and proliferation of hair follicle stem cells in vitro. The positive expression of K19 hair follicle stem cells in 1,3,7 passage recovered from explants were respectively 52.0%±1.62%,68.4%±1.82% and 72.0%±2.42%，the positive expression of integrin-β1 were respectively 52.5%±2.12%,66.3%±1.98% and 73.0%±2.16%，but the positive expression of K19 hair follicle stem cells in 1,3,7 passage from enzyme digestion were respectively 56.2%±3.12%,61.7%±1.17% and 64.0%±3.02%，that of integrinβ1 were 56.0%±112%, 63.0%±1.12% and 68.0%±2.32%. The CFEs of hair follicle stem cells in 1,3 and 7 passage by explants were respectively 18.4% ±0.77%,31.3%±0.88% and 44.7%±1.03%，but the CFEs of hair follicle stem cells in 1,3,7 passage by enzyme digestion were respectively 22.6%±230%,26.9%±0.86% and 32.8%±1.05%；The hair follicle stem cells isolated from explants could be subcultured for 19 passages in vitro but those from enzyme digestion could be passaged just 12 times in the same culture condition. The results showed that hair follicle stem cells can be separated by both methods and the stem cells from explants have higher efficiency in immunohistochemical staining, CFE and subculture ability than those from enzyme digestion when they were subcultured in vitro （P<0.01）.

Optimizing Parameters of Nuclear Transfer on Skin Epidermal Cells of Adult Rabbits

YANG Chun-rong;FENG Xiu-liang;LEI An-min;HUA Jin-lian;GAO Zhi-min;DOU Zhong-ying

2006, 37(5): 441-446. doi:

Abstract ( 1419 )

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This paper screened and optimized some parameters of nuclear transfer on rabbits, including parthenogenesis, fusion, activation, and development of NT embryos, the nuclear donors were skin epidermal cells of adult rabbits. The parthenogenesis rates were no significant difference, under the electric field of 160 V/mm (77.78%), 200 V/mm (95.12%), and 240 V/mm (97.30%); the blastocyst rates under 200 V/mm (52.94%) and 240 V/mm (48.64%) were significantly higher than that under 160 V/mm (16.67%); the fusion rates of NT embryos were no significant difference under 200 V/mm (82.85%) and 240 V/mm (79.78%); the cleavage rate under 200 V/mm (71.69%) was significantly higher than that under 240 V/mm (56.56%), the collapse rate under 200 V/mm (6.82%) was significantly lower than that under 240 V/mm (17.92%). There was about 60% that the first polar body was together with and close to spindle under spindleview imaging system. The cleavage rate with visible pro-nuclears zone (91.75%) was very significantly higher than that without visible pro-nuclears zone (42.86%). The blastocyst rate (24.39%) of NT embryos was lower than developmental rate (86.05%) of embryos of pro-nuclear zygote in vitro, but both blastocyst cells were no significant difference with those (81±17) of spontaneous mating and development in vivo.Implanted 646 2-4-cell NT embryos into 38 synchronized or asynchronized recipients, no fetus was born.

Effect of Elimination of All Anaerobic Fungi on Fibre Degradation in Rumen for Sheep

HUO Xian-xian;HOU Xian-zhi;LI Da-biao;Aoligerima

2006, 37(5): 447-451. doi:

Abstract ( 607 )

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Three Mongolian and han cross-bred rams (30±1.82) kg live weight with a permanent cannula in the rumen were used to investigate the effect of elimination of anaerobic fungi on fibre digestibility of straw with nylon bag methods in sacco. The results showed that the degradation dynamic parameters of different fibre were changed and the degradation rates of DM，NDF, ADF were significantly decreased (P<0.05). Digestibility of NDF, ADF was also significantly decreased (P<0.05), but the degradation rate of DM was no obvious difference. It suggested that the anaerobic fungi chiefly influence the fibre digestion.

Cysteamine Promotes Development of Mammary Gland in Dairy Goats

WANG Yue-ying;WANG Yan-ling;YANG Guo-yu;FAN Guang-li

2006, 37(5): 452-456. doi:

Abstract ( 723 )

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Six local nonpregnant dairy goats were used in a self-control design and eight pregnant dairy goats were used in a match design to study the effects of CS on mammary development in dairy goats.The results showed that the alveolar and ductal epithelial of non-pregnant dairy goats in control period were less than that in experiment period.With pregnant processing,the mammary gland of pregnant dairy goats also developed little by little,but the alveolar and ductal epithelial of pregnant dairy goats in experiment group were in abundance in comparison with the control group.As well as CS also enhanced the levels of the related hormone in plasma with mammary gland development.The above results suggested that CS promoted mammary gland development in dairy goats to some extent.

Effect of Epidermal Growth Factor, Glutamine and pGRF Gene Plasmid on the Intestinal Development of Early Weaning Piglets

FAN Zhi-yong;WANG Kang-ning;ZHOU Ding-gang

2006, 37(5): 457-463. doi:

Abstract ( 711 )

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An experiment was conducted to study the effect of epidermal growth factor(EGF),glutamine(Gln) and porcine growth release factor gene plasmid(pGRF gene plasmid)on the development of the digestive system in weaned piglets. In this trial, thirty piglets(Landrace × Large white × Taihu, initial weight 4.51 kg),weaned at 28 days, were randomly allocated to five treatment, three replicates of two piglets each. Pigs in different group were given different combination of the substances metioned above. Group I was given EGF alone; Group Ⅱ was given EGF and Gln; Group Ⅲ was given EGF and pGRF gene plasmid; Group Ⅳ was given EGF, Gln and pGRF gene plasmid; Group Ⅴ was given physiological saline as control. Samples were collected 21 days later. The weight of main digestive organs including stomach, liver, pancreas, small intestine and mucous of intestine were measured. The length of villi, the value of ALP in mucous of small intestine and the value of bacterial shift were detected. The results showed that the effect of the combined of EGF, Gln and pGRF gene plasmid was much better than that of EGF. Compared with control group, the weight of small intestine, frontal jejunum, middle jejunum, rear jejunum and entire jejunum of group Ⅳ were inereased by 37.12%(P<0.1),103%(P<0.05),8627%(P<0.05),100%(P<0.1) and 96.02%(P<0.05) respectively. In group Ⅳ, the length of villi in jejunum was increased by 89.68% and no bacterial translation was found. But the activity of alkaline phosphate enzyme wasn’t influnced significantly in every treated groups （P＞0.05）.

Comparison of Kjeldahl and Dumas Combustion Methods for Determination of Nitrogen Content in Feedstuffs

GUO Wang-shan;MENG Qing-xiang

2006, 37(5): 464-468. doi:

Abstract ( 717 )

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This study was conducted to examine the difference of the N content of 22 N-containing materials, including 5 roughage feeds, 6 high energy feeds and 9 protein feedstuffs, and 2 reference compounds, determined by Kjeldahl (Foss Model 2300, Sweden) and Dumas combustion ( Rapid N III, Elementar，Germany), respectively. Eleven Ncontaining feed samples were dispensed to 3 individual laboratories for N analysis by the two methods，to ensure a quick and no polluted method which can determine the nitrogen content in feedstuffs. The result showed that there was a linear correlation (r =0.999 8，P<0.001) of N contents between the two determination methods. Most protein and high energy feedstuffs had no significant difference(P>0.05,CV<5%), but a significant difference（P<0.05，CV<5%）was obtained in 4 roughage feedstuffs. With the Kjeldahl determination, the N content of 7 samples were significantly different (P<0.05) among 3 laboratories, having more than 5% of CV for 2 samples. Unlike the Kjeldahl method, however, the Dumas combustion method produced a significant difference (P<0.05) in the N determination of only 2 samples, with less than 5% of CV values for all feed samples among the 3 laboratories. The N content CV values obtained from the 3 laboratories in 11 samples were higher (P<0.01) with Kjekldahl method than those with Dumas combustion method. It was concluded that Dumas method can be used as a regular method for determination of nitrogen contents in feedstuffs or diets.

Expression and Active Detection of the Gene Encoding the FMDV Structural Protein VP1 in Transgenic Arabidopsis thaliana

PAN Li;ZHANG Yong-guang;WANG Yong-lu;FANG Yu-zhen;JIANG Shou-tian;WANG Bao-qin;WANG Wen-xiu;WANG Wei;SUN Yuan;L Jian-liang;XIE Qing-ge

2006, 37(5): 469-473. doi:

Abstract ( 736 )

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The plant seed-specific expression vector p7SBin438/VP1 for VP1 gene of footandmouth disease virus O/China/99 strain was constructed. Mediated with Agrobacterium tumefaciens GV3101 harboring p7SBin438/VP1, VP1 gene was transferred into Arabidopsis thaliana via floral dip. After selecting by Kanamysin, a number of resistant lines were obtained. The presence of VP1 gene in the transformed Arabidopsis thaliana genome was confirmed by PCR. The active detection of expressed target protein in the pods and leaves of transgenic Arabidopsis thaliana was confirmed by sandwich-ELISA and Western blot. Genetic analysis of transgenic progeny was performed by PCR. Experimental results showed that the plant seed-specific expression vector was successfully constructed and the structural protein VP1 was expressed in pods at high-level. Western blot showed that recombinant protein expressed in pods had immunocompetence and could react with FMDV antisera. Progeny test showed that VP1 gene in the transgenic Arabidopsis thaliana was heritable stablely. These results may provide the experimental bases for further research on transformed leguminous plant.

Establishment of Antigen-captured ELISA Using Monoclonal Antibodies to Detect Classical Swine Fever Virus

LIU Jianwen;YU Xinglong;ZHANG Liying;TU Changchun

2006, 37(5): 474-479. doi:

Abstract ( 695 )

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The five monoclonal antibodies (mAbs) against classical swine fever virus were generated by immunization of BALB/c mice respectively with CSFV DNA vaccine plasmid (expressing glycoprotein E2) and E.coli-expressed E2 protein. These mAbs could react with CSFV growing in PK-15 cells in indirect immunofluorescent assay (IFA), as well as with expressed E2 protein in western-blotting. Of them, the mAbs 1E2, 3B7 and 4B6 are CSFV specific antibody without cross reaction with E2 protein of BVDV, therefore have been used, along with rabbit anti-CSFV IgG, to develop the antigen-captured ELISA(AC-ELISA) for detecting CSFV antigen directly from clinical tissue samples. The cut-off value was determined by testing negative tissue samples of 24 healthy pigs. The method was testified by comparison with virus isolation and RT-PCR of 31 positive tissue samples from piglets experimentally infected with CSFV shimen strain,11 clinically CSF cases and 12 healthy samples. The results showed that the AC-ELISA is sensitive, specific and has high consistency with virus isolation and RT-PCR.

mRNA Differential Display PCR of Maduramisin Sensitive and Resistant Eimeria tenella Strain

AN Jian;WANG Ming;FAN Bao-liang;ZHAO Xing-bo; LI Ning

2006, 37(5): 480-484. doi:

Abstract ( 683 )

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The differential expression between merozoites of maduramisin sensitive and maduramisin resistant strains of E. tenella was detected with the technique of random mRNA Differential Display. The 34 differential DNA fragments were identified by reverse Northern dot hybridization. Four fragments were found to be really different, the differential expression fragments were cloned into pMD-18T vector and sequenced. Then the possible functions and the relationship of the 4 differential DNA fragments to drug resistance were predicted with Blast. Fragment ACD3-2 was found to be similar to Eimeia tenella microneme-5 gene, which is related to the parasite invasion, moving; Fragment HCD1 similar to part sequence of Eimeia tenella surface antigen-17, 16 genes, the other 2 differential expression sequences maybe new genes because no homologous gene were found in GenBank by Blast analysis.

Studies on Biological Characters of Cryptosporidium meleagridis from Quail

SUN Ming-fei;ZHANG Long-xian;NING Chang-shen;JIAN Fu-chun;ZHAO Jin-feng;WANG Rong-jun;LIU Hong-ying

2006, 37(5): 485-491. doi:

Abstract ( 681 )

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A strain of Cryptosporidium derived from quail was identified as C.meleagridis in previous experiment.The pathogenicity of the Cryptosporidium strain to ostriches,chickens,ducks and mice were studied,these experimental animals were divided into infective group, immunosupressive group and control group respectively. There were only diarrhea in quail, chicken, mice, ducks which infected with C.meleagridis, but no clinical signs in ostriches.There were multiple peaks in those immunosuppressive experimental animals. The experiments were carried out on pathology of some organs in young chickens and mice that infected with Cryptosporidium meleagridis from quail, we found that Cryptosporidium derived from quail mainly parasitized in duodenum of mice and ileum of chicken which challenged with it, the intestinal villi of these experimental animals became atrophied and desquamated.A few parasites were found in ileum of duck.The results suggest that the duck is a new host of C.meleagridis from quail, and the ostrich can′t be infected by this C.meleagridis. Parasitic site of C. meleagridis from quail in quail, chicken,duck were different from that of mice.

Study on Some Biological Characters of Zmu-1：DHP Guinea Pig

LIU Di-wen

2006, 37(5): 492-495. doi:

Abstract ( 659 )

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To supply the biological data of Zmu-1：DHP guinea pig，the fragmentation reaction of the sensitized mast cell,the activity of total complement,the phagocytic and oxidative burst function of polymorphonuclear leukocytes,the sensitivity to FMDV in different guinea pig were studied by attacking mast cell by antigen,complement CH50,LCM and virus infection．The results showed that the fragmentation proportion of the mast cell in Zmu-1：DHP guinea pig was significantly lower than other strain（P<0.01），the content of complement of the strain was higher（P<0.01），the phagocytic rate of polymorphonuclear leukocytes of the strain was higher（P<0.05），but the oxidative burst activity of the strain was lower（P<0.01）than that of others. And 100% sensitivity to FMDV in Zmu-1：DHP guinea pig was presented．It is indicated that the strain appears the new biological character，and possesses the higher value in application．

Studies on the Dynamic Changes of the Level of IL-2，IFN-γ and TNF-α in Porcine Serum after Heat Stress

HU Yan-xin;SHE Rui-ping;ZHANG Hong-yu;GUO Yan-jun;XIAO Chong;LIU Feng-hua

2006, 37(5): 496-499. doi:

Abstract ( 1070 )

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The effect of heat stress on the level of IL-2,IFN-γ and TNF-α in porcine serum were investigated in this study.Twelve healthy three-month-old pigs were divided into two groups at random, heat stress group and control group. The two groups were put into different AGCs(artificial climate chambers), one of which simulated high temperature in summer (the temperature changed from 26 ℃ to 39 ℃ and lasted four hours at 39 ℃ ), the other always keep at 22 ℃. The experiment lasted ten days and in the 1st, 3rd, 5th,7th and 9th day , 5 mL blood were drawn out of jugular vein soon after 39 ℃ heat stress and level of IL-2,IFN-γ and TNF-α in porcine serum was determined by using ELISA. Contrast with the control group, the level of IL-2 in porcine serum of heat stress group increased dramatically (P<0.05) on the 1st and the 3rd day after heat stress; The level of IFN-γ in porcine serum decreased dramatically (P<0.05) on the 3rd and the 5th day ; The level of TNF-α in porcine serum of heat stress group increased,but not dramatically. With the experiment going, the level of IL-2,IFN-γ and TNF-α in porcine serum of heat stress group increased or decreased gradually. On the 10th day, the level of these cytokines of the two groups was similar. The data presented indicated that heat stress restrained immune functions and the conclusion was in accordance with forepassed reports.

Determination of Residual Avermectins in the Cattle Liver Using HPLC with Fluorescence Detection

HOU Xiao-lin;HE Ji-hong;DU Xiang-dang;SHEN Jian-zhong

2006, 37(5): 500-503. doi:

Abstract ( 1312 )

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A method using high-performance liquid chromatography (HPLC) with fluorescence detection was presented for the simultaneous measurement of the eprinomectin, avermectin, doramectin and ivermectin in liver. Samples were extracted with acetonitrile and cleaned up using solid phase extraction on alumina-B and C₁₈ column, and followed by derivatization with acetic anhydride and 1-methylimidazole in acetonitrile. The optimum time required for the completion of derivation reaction of the 4 drugs was 100 min at 96℃. Mean recoveries of the 4 drugs in bovine liver ranged from 70.02% to 88.75%. The intra-and inter-day variations were less than 8.52% and 7.13% respectively. The limit of detection was 0.4-0.5 μg/kg, and the limit of quantitation of the method was 2 μg/kg for all of the 4 drugs. The procedure provides a reliable and sensitive method for routine residual analysis.

An Survey of Dermatophytes on Hair of Dog and Cat in Beijing

DING Ming-xing;LIN De-gui

2006, 37(5): 504-509. doi:

Abstract ( 1225 )

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For investigating carrier rate of dermatophytes on hair of dog and cat in Beijing, samples from seven points of the pets were used to cultivate fungus on Sabouraud medium / Dermatophyte Test Medium(DTM) at 26 ℃ for two weeks. A total of 770 hair samples from 78 dogs and 32 cats were cultivated to isolate fungus from January 2002 to March 2003 in the veterinary teaching hospital of CAU. Identification was made by colony morphology and microscopic structures.The results indicated that the carrier rate was low (2.78%,1/36) in dog, but was high (9.4%,3/32)in cat in Beijing. The distributing pattern of saprophytes on hairs was as follows, highest carrier rate was found inside the ear, next the right carpus, the lateral corner of right eye, the external of ear base, the back, the tail and the abdomen. The main saprophytes on hair of dogs were: 30.69% Penicillium, 12.64%Alternaria, 6.14% Aspergillus, 5.42% Mucor, 4.69% Cladosporum, 20.94% Candida. 9.39% fungi could not be identified. 67 strains of bacteria were also isolated. Other pathogenic dermatomyces were cultured from pets, they were Malassezia (Pityrosporium) furfur, Piedraia hortae, Blastomyces dermatitidis, Coccidioides immitis, Paracoccidioides brasiliensis and Scopularis brevicaulis. This suggests that pet might have been infected by them cutaneously or systemically.

Polymorphism, Effect and Linkage Analysis of BMPR-ⅠB Gene in Small Tailed Han Sheep

YIN Zi-hui;JIANG Yun-liang;FAN Xin-zhong;WANG Yu;TANG Hui;YUE Yong-sheng

2006, 37(5): 510-513. doi:

Abstract ( 672 )

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The Q249R mutation (A746G) in bone morphogenetic protein receptor-ⅠB (BMPR-ⅠB) is associated with the high prolificacy of Booroola Merino sheep. In our previous study, we found that, in Small Tailed Han sheep population, a high proportion of ewes carry such mutation. The present study further analyzed the polymorphism, effect and linkage of BMPR-ⅠB gene in this breed. The results indicated that the genotype frequencies of BB, B+ and ++ were 0.541 8, 0.364 5 and 0.093 7 respectively, and the allelic frequency of B was 0.724 1.The substitution effect and dominance effect of the B allele were estimated to be 0.46 and 0.16 for the first parity and 0.56 and 0.11 for the second parity, respectively. Moreover, the fully FecB-associated microsatellite marker OARJL36 was genotyped in the BB ewes of Small Tailed Han sheep. We found that, in Small Tailed Han sheep, recombination between BMPR-ⅠB gene and OARJL36 locus are still occurring.

Clone and Phylogenetic Analysis of S1 Gene of Coronavirus Isolated from Pigeon in China

QIAN Hua-dong;WU Zu-li;ZHU Jian-guo;HUA Xiu-guo

2006, 37(5): 514-517. doi:

Abstract ( 1079 )

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The S1 gene of the pigeon original coronavirus (PSH) which was first isolated and identified in China was amplified by reverse transcription-polymerase chain reaction (RT-PCR) method, then it was cloned and sequenced. The results showed that the S1 gene of PSH was 1 626bp and encoded a polypeptide of 541 amino acids. The cleavage site sequence of S1 protein of PSH contains five consecutive basic amino acids, namely, Arg-Arg-Phe-Arg-Arg which is similar to the most IBV strains. Sequence data demonstrated that the complete S1 amino acid of PSH shared 75.0%-99.6% similarity with the published S1 sequences of M41,H52,H120,Beau,Gray,Holte,Conn,SH1,SH2,SH5 and SH6 IBV strains.But it only shared 24.7%,25% similarity with TCV Gh and G1 strain respectively. Based on these findings, the virus has been tentatively identified as a novel pathogenic field IBV strain, generated through recombination.

Sequence and Molecular Characterization of 16S rRNA Gene of Eperythrozoon wenyonii-Hubei Strain

FU Yuan;LI Shu-qing;DU Kai;LIU Qin;ZHOU Yan-qin;LIU En-yong;YAO Bao-an;ZHAO Jun-long

2006, 37(5): 518-522. doi:

Abstract ( 1020 )

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The complete 16S rRNA gene of E.wenyonii-Hubei strain was determined by PCR. It was sequenced and blasted. The results showed that the sequence was 98.7% identical to that of Neimark published sequence（AF016546）. The 1 471bp complete sequence was compared with the sequence of 5 mycoplasmas,14 haemotrophic bacteria and Anaplasma marginale available in GenBank. The data were analyzed and a phylogenetic tree was established. The results indicated that the hereditary distance of E.wenyonii was close to that of pneumoniae group，and the hereditary distance was far from Anaplasma marginale.

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