Abstract: Goat liver cDNA library was constructed, and the DNAs of phage were isolated by plate lysate method. The UK114 gene was amplified by polymerase chain reaction , using two primers.and then were cloned into pGEM-T easy vector. Sequencing of the PCR-derived fragments showed that the UK114 cDNA consists of 1 017 bp. The 5′ non-coding region consists of 39 bp, the 3′ non-coding region consists of 567 bp, and its coding region is about 411 bp (40 nt450 nt), which can encode 137 amino acid (14.2ku). Compared this product with UK114 that Colombo reported, its 5′ non-coding region consists of 102 bp, and there are 9 bp identical. Their encoding region consists of 411 bp, and there is only one mutation nucleotide in coding region, and it is meaningless. The 3′ non-coding consists of 522 bp, the difference between them is that the UK114 of Colombo reported is the poly A tails and the UK114 is the different nucleotides. The homologous was 91%.There are 86 mutation nucleotides, and they are all meaningless. There is only one mutation nucleotide in coding region.