ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2006, Vol. 37 ›› Issue (7): 635-639.doi:

• 遗传繁育 • Previous Articles     Next Articles

Cloning of Porcine ERα Gene E Domain Partial cDNA and Expression in E. coli System

DING Li-jun; WU Jin-sheng; WANG Jian-wu; DING Jia-tong   

  1. 1. College of Animal Science and Technology,Yangzhou University, Yangzhou 225009,China;2. Jiangsu Polytechnic College of Agriculture and Forestry, Jurong 212400,China;3. College of Biology, China Agricultural University, Beijing 100094,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-07-25 Published:2006-07-25

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Abstract:

Porcine ERα gene E domain partial cDNA 546 bp, was amplified from Meishan pig uterus by RT-PCR method and recombined into pGEX-6p-1 plasmid expressing glutathione S-transferase (GST) fusion protein. After identified by the restriction enzyme digestion and sequencing, the recombinant clones were transformed into the competent expressive cells of E.coli BL21. Recombinant E.coli was induced by IPTG and GST-ERαE fusion protein with molecular weight of 49 ku was obtained. Scanning analysis indicated that the fusion protein amounted up to 32% of the total bacterial protein expressed. The results showed that pGEX-6p-1-ERαE recombinant vector was successfully constructed and GST-ERαE fusion protein was highly efficient expressed.