Abstract: In this study, two SNPs of chicken FASN gene were chosen to analyze genetic diversity and their associations with chicken growth and carcass traits by using a F2 designed resource population. Results showed that: (1) In the PCRRFLP site of HaeⅢ, allelic frequencies differed much among different chicken breeds, as B frequencies are the highest in Red Jungle Fowl (0.85), and the lowest in Taihe and Yugan Black (both 0.21). In the PCRRFLP site of PstⅠ, A frequencies are the highest in Chongren Partridge (0.5), and the lowest in Naked Neck chicken (0.19), and the difference of gene frequencies was small among chicken breeds. (2) In HaeⅢ site, it was significantly associated with most growth traits, individuals with CC genotype had the lowest body weight; No significantly difference was found at PstⅠsite; When considering combined genotypes of the two SNPs, individuals with (BC/GG) genotype had the highest body weight. (3) In HaeⅢ site, individuals with BB genotype had the higher fat deposition capacity than that of others, however no significant differences in fat deposition were observed among different genotypes in Pst Ⅰsite; And, when considering combined genotypes of the two SNPs, individuals with (BC/GG) genotype had the highest fat deposition capacity. (4) There were certain interactional effects within the two SNPs including bodyweight and fat deposition traits.