ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2006, Vol. 37 ›› Issue (5): 480-484.doi:

• 预防兽医 • Previous Articles     Next Articles

mRNA Differential Display PCR of Maduramisin Sensitive and Resistant Eimeria tenella Strain

AN Jian; WANG Ming; FAN Bao-liang; ZHAO Xing-bo; LI Ning
  

  1. 1. Department of Animal Science and Technology, Beijing Agricultural College, Beijing 102206, China; 2. College of Veterinary Medicine, China Agricultural University, Beijing 100094, China;3.College of Biotechnology, China Agricultural University, Beijing 100094,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-05-25 Published:2006-05-25

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Abstract: The differential expression between merozoites of maduramisin sensitive and maduramisin resistant strains of E. tenella was detected with the technique of random mRNA Differential Display. The 34 differential DNA fragments were identified by reverse Northern dot hybridization. Four fragments were found to be really different, the differential expression fragments were cloned into pMD-18T vector and sequenced. Then the possible functions and the relationship of the 4 differential DNA fragments to drug resistance were predicted with Blast. Fragment ACD3-2 was found to be similar to Eimeia tenella microneme-5 gene, which is related to the parasite invasion, moving; Fragment HCD1 similar to part sequence of Eimeia tenella surface antigen-17, 16 genes, the other 2 differential expression sequences maybe new genes because no homologous gene were found in GenBank by Blast analysis.